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Inhibition of the Growth of Staphylococcus Aureus by Using Some Antibiotics Produced from Bacillus Subtilis

Yıl 2021, Cilt: 16 , 231 - 239, 31.12.2021
https://doi.org/10.55549/epstem.1068617

Öz

Bacillus subtilis can produce many substances that are considered as a means of defense in addition to being tested and approved as antibiotics. The goal of this study was to see if Bacillus subtilis crude bacteriocin had any antibacterial effects on Staphylococcus aureus .The peptidic bacteriocin is considered of importance in the industrial and medical fields as an anticancer.We got our bacteria from the Bacterial Bank at the College of Science and the Department of Biology. Identification of the isolates was confirmed by microscopically and biochemical tests which include catalase, oxidase, IMViC, blood hemolysis, starch hydrolysis for Bacillus, and growth on Mannitol Salt Agar (MSA), Voges-Jenson agar(V.J), and Coagulase test for Staphylococcus. Based on the capacity of bacteriocin to diffuse in agar, the reverse technique, which eliminated the contact between the producers and sensitive strains, was used to detect bacteriocin antibacterial action. Results demonstrate the antibacterial effect of Bacillus subtilis bacteriocin by the inhibition of Staphylococcus aureus as compared to other indicator isolates.

Kaynakça

  • Abriouel, H., Franz, C. M., Omar, N. B., & Gálvez, A. (2011). Diversity and applications of Bacillus bacteriocins. FEMS Microbiology Reviews, 35(1), 201-232.
  • Agarwal, S., Sharma, G., Dang, S., Gupta, S., & Gabrani, R. (2016). Antimicrobial peptides as anti-infectives against Staphylococcus epidermidis. Medical Principles and Practice, 25(4), 301-308.
  • Al-Dahbi, A. M., & Al-Mathkhury, H. J. (2013). Distribution of methicillin resistant Staphylococcus aureus in Iraqi patients and healthcare workers. Iraqi Journal of Science, 54(2), 293-300.
  • Bierbaum, G., & Sahl, H. G. (2009). Lantibiotics: mode of action, biosynthesis and bioengineering. Current Pharmaceutical Biotechnology, 10(1), 2-18.
  • Boyce, J. M., Cookson, B., Christiansen, K., Hori, S., VuopioVarkila, J., Kocagaz, S. Oztop, A. Y., Vandenbroucke-Grauls, C.M.J .E., Harbarth, S., and Pittet, D. (2005 . ( Meticillin-resistant S. aureus. The Lan. Infect. Disea, 5(10), 653-663
  • Chopra, L., Singh, G., Jena, K. K., & Sahoo, D. K. (2015). Sonorensin: a new bacteriocin with potential of an anti-biofilm agent and a food biopreservative. Scientific Reports, 5(1), 1-13.
  • Cintas, L. M., Rodriguez, J. M., Fernandez, M. F., Sletten, K., Nes, I. F., Hernandez, P. E., & Holo, H. (1995).
  • Isolation and characterization of pediocin L50, a new bacteriocin from Pediococcus acidilactici with a broad inhibitory spectrum. Applied and Environmental Microbiology, 61(7), 2643-2648.
  • Cleveland, J., Montville, T. J., Nes, I. F., & Chikindas, M. L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of Food Microbiology, 71(1), 1-20. Cotter, P. D., Hill, C., & Ross, R. P. (2005). Bacteriocins: developing innate immunity for food. Nat. R .Microbiol., 3(10), 777-788.
  • Cotter, P. D., Ross, R. P., & Hill, C. (2013). Bacteriocins—a viable alternative to antibiotics?. Nature Reviews Microbiology, 11(2), 95-105.
  • Croft, A. C., D'Antoni, A. V., & Terzulli, S. L. (2007). Update on the antibacterial resistance crisis. Medical Science Monitor, 13(6), 103-118. Dubnau, D. (1982). The molecular biology, of the bacilli, volume i: bacillus subtilis. The Public Health Research. Deurenberg, R. H., & Stobberingh, E. E. (2008).
  • The evolution of Staphylococcus aureus. Infection, Genetics and Evolution, 8(6), 747-763. Hammami, R., Fernandez, B., Lacroix, C., & Fliss, I. (2013).
  • Anti-infective properties of bacteriocins: an update. Cellular and Molecular Life Sciences, 70(16), 2947-2967. Hashemizadeh, Z., Bazargani, A., & Davarpanah, M. A. (2011).
  • Blood culture contamination in a neonatal intensive care unit in Shiraz, Southwest-Central Iran. Medical Principles and Practice, 20(2), 133-136. Kareem, S. M., Al-Jubori, S. S., & Ali, M. R. (2015).
  • Prevalence of erm genes among methicillin resistant Staphylococcus aureus MRSA Iraqi isolates. Int J Curr Microbiol Appl Sci, 4(5), 575-585.
  • Koneman, E. W., Allen, S. D., Janda, W. M., Schreckenberger, P. C., & Winn, W. C. (1997). Diagnostic microbiology. In The nonfermentative gram-negative bacilli (pp. 253-320).
  • Lippincott-Raven Publishers. Mulvey, M. R., & Simor, A. E. (2009). Antimicrobial resistance in hospitals: how concerned should we be?. Cmaj, 180(4), 408-415.
  • Nicholson, W. L. (2002). Roles of Bacillus endospores in the environment. Cellular and Molecular Life Sciences CMLS, 59(3), 410-416.
  • Nicolle, P., & Prunet, J. (1964). La propriete colicinogene dans lespece salmonella typhi. In Annales De L Institut Pasteur. Masson Editeur. O’sullivan, L., Ross, R. P., & Hill, C. (2002).
  • Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochimie, 84(5-6), 593-604.
  • Schwalbe, R., Steele-Moore, L., & Goodwin, A. C. (Eds.). (2007). Antimicrobial susceptibility testing protocols. Crc Press.
  • Snyder, A. B., & Worobo, R. W. (2014). Chemical and genetic characterization of bacteriocins: antimicrobial peptides for food safety. Journal of the Science of Food and Agriculture, 94(1), 28-44.
Yıl 2021, Cilt: 16 , 231 - 239, 31.12.2021
https://doi.org/10.55549/epstem.1068617

Öz

Kaynakça

  • Abriouel, H., Franz, C. M., Omar, N. B., & Gálvez, A. (2011). Diversity and applications of Bacillus bacteriocins. FEMS Microbiology Reviews, 35(1), 201-232.
  • Agarwal, S., Sharma, G., Dang, S., Gupta, S., & Gabrani, R. (2016). Antimicrobial peptides as anti-infectives against Staphylococcus epidermidis. Medical Principles and Practice, 25(4), 301-308.
  • Al-Dahbi, A. M., & Al-Mathkhury, H. J. (2013). Distribution of methicillin resistant Staphylococcus aureus in Iraqi patients and healthcare workers. Iraqi Journal of Science, 54(2), 293-300.
  • Bierbaum, G., & Sahl, H. G. (2009). Lantibiotics: mode of action, biosynthesis and bioengineering. Current Pharmaceutical Biotechnology, 10(1), 2-18.
  • Boyce, J. M., Cookson, B., Christiansen, K., Hori, S., VuopioVarkila, J., Kocagaz, S. Oztop, A. Y., Vandenbroucke-Grauls, C.M.J .E., Harbarth, S., and Pittet, D. (2005 . ( Meticillin-resistant S. aureus. The Lan. Infect. Disea, 5(10), 653-663
  • Chopra, L., Singh, G., Jena, K. K., & Sahoo, D. K. (2015). Sonorensin: a new bacteriocin with potential of an anti-biofilm agent and a food biopreservative. Scientific Reports, 5(1), 1-13.
  • Cintas, L. M., Rodriguez, J. M., Fernandez, M. F., Sletten, K., Nes, I. F., Hernandez, P. E., & Holo, H. (1995).
  • Isolation and characterization of pediocin L50, a new bacteriocin from Pediococcus acidilactici with a broad inhibitory spectrum. Applied and Environmental Microbiology, 61(7), 2643-2648.
  • Cleveland, J., Montville, T. J., Nes, I. F., & Chikindas, M. L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of Food Microbiology, 71(1), 1-20. Cotter, P. D., Hill, C., & Ross, R. P. (2005). Bacteriocins: developing innate immunity for food. Nat. R .Microbiol., 3(10), 777-788.
  • Cotter, P. D., Ross, R. P., & Hill, C. (2013). Bacteriocins—a viable alternative to antibiotics?. Nature Reviews Microbiology, 11(2), 95-105.
  • Croft, A. C., D'Antoni, A. V., & Terzulli, S. L. (2007). Update on the antibacterial resistance crisis. Medical Science Monitor, 13(6), 103-118. Dubnau, D. (1982). The molecular biology, of the bacilli, volume i: bacillus subtilis. The Public Health Research. Deurenberg, R. H., & Stobberingh, E. E. (2008).
  • The evolution of Staphylococcus aureus. Infection, Genetics and Evolution, 8(6), 747-763. Hammami, R., Fernandez, B., Lacroix, C., & Fliss, I. (2013).
  • Anti-infective properties of bacteriocins: an update. Cellular and Molecular Life Sciences, 70(16), 2947-2967. Hashemizadeh, Z., Bazargani, A., & Davarpanah, M. A. (2011).
  • Blood culture contamination in a neonatal intensive care unit in Shiraz, Southwest-Central Iran. Medical Principles and Practice, 20(2), 133-136. Kareem, S. M., Al-Jubori, S. S., & Ali, M. R. (2015).
  • Prevalence of erm genes among methicillin resistant Staphylococcus aureus MRSA Iraqi isolates. Int J Curr Microbiol Appl Sci, 4(5), 575-585.
  • Koneman, E. W., Allen, S. D., Janda, W. M., Schreckenberger, P. C., & Winn, W. C. (1997). Diagnostic microbiology. In The nonfermentative gram-negative bacilli (pp. 253-320).
  • Lippincott-Raven Publishers. Mulvey, M. R., & Simor, A. E. (2009). Antimicrobial resistance in hospitals: how concerned should we be?. Cmaj, 180(4), 408-415.
  • Nicholson, W. L. (2002). Roles of Bacillus endospores in the environment. Cellular and Molecular Life Sciences CMLS, 59(3), 410-416.
  • Nicolle, P., & Prunet, J. (1964). La propriete colicinogene dans lespece salmonella typhi. In Annales De L Institut Pasteur. Masson Editeur. O’sullivan, L., Ross, R. P., & Hill, C. (2002).
  • Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochimie, 84(5-6), 593-604.
  • Schwalbe, R., Steele-Moore, L., & Goodwin, A. C. (Eds.). (2007). Antimicrobial susceptibility testing protocols. Crc Press.
  • Snyder, A. B., & Worobo, R. W. (2014). Chemical and genetic characterization of bacteriocins: antimicrobial peptides for food safety. Journal of the Science of Food and Agriculture, 94(1), 28-44.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Yusra Yahya Agha

Yayımlanma Tarihi 31 Aralık 2021
Yayımlandığı Sayı Yıl 2021Cilt: 16

Kaynak Göster

APA Agha, Y. Y. (2021). Inhibition of the Growth of Staphylococcus Aureus by Using Some Antibiotics Produced from Bacillus Subtilis. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 16, 231-239. https://doi.org/10.55549/epstem.1068617