Isolation and Studying the Effect of Protein Fractions of Red chili pepper (Capsicum annuum L.) on Some Biochemical Parameters in Experimental Diabetic Rats

Zena A.m. Aljawadı

Research Article

Year 2019, Volume: 7 , 289 - 295, 24.11.2019

Zena A.m. Aljawadı

Abstract

References

M. K. Hwang, A. M. Bode, S. Byun, N. R. Song, H. J. Lee, K. W. Lee and Z. Dong. Cancer Research. 70 (17): 6859–6869, 2010. J. J. Tewksbury and G. P. Nabhan. Nature., 412 (6845): 403–404. 2001. KD. Ahuja and MJ. Ball. Br J Nutr., 96(2): 239-242. 2006. KD. Ahuja, IK. Robertson and DP. Geraghty. Am J Clin Nutr., 84(1): 63-69. 2006. SK. Sharma and AS. Vij. Pharmacol Eur J., 720: 55-62, 2013. H. Passing and W. Bablok. J. Clin. Chem. Clin. Biochem., 24: 863-869, 1983. N.W. Tietz. Text book of clinical chemistry,3rd ed .C.A. Burtis, E.R. Ashwood, W.B. Saunders, pp.809-856,1999. Chabral and Chardonnet , Cited by Geloson Torol, Philip G. Ackermann, Drac. Clin. Chem. Little, Brown and Company, Boston, 1975. R.G. Steel and J.H. Torrie. Principles and Procedures of Statistics a Biometrical Approach. 2nd ed., McGraw-Hill, Inc., Singapore, 1980. M.M. Abdel Magied, N.A. Salama and M.R. Ali. Journal of Food and Nutrition Research, 2(11): 850-856, 2014. L. Nordquist, R. Brown, A. Fasching, P. Persson and F. Palm. Am. J. Physiol. Renal Physiol., 297(5): F1265–1272, 2009. K. Esayas, A. Shimelis, F. Ashebir and R. Negussie. Bulletin of the Chemical Society of Ethiop, 25(3): 451-454. 2011. Y.T. Liang, X. Tian, J.N. Chen, C. Peng, K.Y. Ma, Y. Zuo, R. Jiao, Y. Lu, Y. Huang and Z. Chen. European Journal of Nutrition., 52(1): 379-388, 2013. M. Ozgur1, T. Ozcan, A. Akpinar-Bayizit and L. Yilmaz-Ersan. African Journal of Agricultural Research., 6 (25): 5638-5644, 2011. R. Chandini, V. Udayabhaskaran, J.P. Binoy and K.P. Ramamoorthy. International Journal of Diabetes in Developing Countries, 33(2): 83–85, 2013.

Isolation and Studying the Effect of Protein Fractions of Red chili pepper (Capsicum annuum L.) on Some Biochemical Parameters in Experimental Diabetic Rats

Year 2019, Volume: 7 , 289 - 295, 24.11.2019

Zena A.m. Aljawadı

Abstract

The research was an attempt to Isolate and study the active protein compounds from the aqueous extract of red chili pepper (Capsicum annuum L.), using different biochemical techniques. Two compounds (A and B) were isolated using gel filtration chromatography for the precipitate produced by ammonium sulphate precipitation. The results predicted that, glucose level was lowered by intraperitoneally administration of the concentrated aqueous extract and the protein compound (B) compared with oral administration. The comparative molecular weight of the isolated protein active compound (peak B), was found to be 24000 Dalton, also it included the effect of the aqueous extract and the protein compound (B) in four doses (50, 75, 100 and 125 mg/kg body weight) on certain blood constituents in normal and alloxan induced diabetic rats. In normal rats, the blood glucose level was significantly lowered by crude aqueous extract and the protein compound in a dose (75 mg/kg body weight) compared with control group. and in a same dose showed a significant decrease in serum cholesterol and total lipids level. In diabetic rats, the protein compound in a dose of (75mg/kg body weight) showed a significant decrease in serum glucose, cholesterol and total lipids levels.

Keywords

Red chili pepper , (Capsicum annuum L.) , Diabetes disease

References

M. K. Hwang, A. M. Bode, S. Byun, N. R. Song, H. J. Lee, K. W. Lee and Z. Dong. Cancer Research. 70 (17): 6859–6869, 2010. J. J. Tewksbury and G. P. Nabhan. Nature., 412 (6845): 403–404. 2001. KD. Ahuja and MJ. Ball. Br J Nutr., 96(2): 239-242. 2006. KD. Ahuja, IK. Robertson and DP. Geraghty. Am J Clin Nutr., 84(1): 63-69. 2006. SK. Sharma and AS. Vij. Pharmacol Eur J., 720: 55-62, 2013. H. Passing and W. Bablok. J. Clin. Chem. Clin. Biochem., 24: 863-869, 1983. N.W. Tietz. Text book of clinical chemistry,3rd ed .C.A. Burtis, E.R. Ashwood, W.B. Saunders, pp.809-856,1999. Chabral and Chardonnet , Cited by Geloson Torol, Philip G. Ackermann, Drac. Clin. Chem. Little, Brown and Company, Boston, 1975. R.G. Steel and J.H. Torrie. Principles and Procedures of Statistics a Biometrical Approach. 2nd ed., McGraw-Hill, Inc., Singapore, 1980. M.M. Abdel Magied, N.A. Salama and M.R. Ali. Journal of Food and Nutrition Research, 2(11): 850-856, 2014. L. Nordquist, R. Brown, A. Fasching, P. Persson and F. Palm. Am. J. Physiol. Renal Physiol., 297(5): F1265–1272, 2009. K. Esayas, A. Shimelis, F. Ashebir and R. Negussie. Bulletin of the Chemical Society of Ethiop, 25(3): 451-454. 2011. Y.T. Liang, X. Tian, J.N. Chen, C. Peng, K.Y. Ma, Y. Zuo, R. Jiao, Y. Lu, Y. Huang and Z. Chen. European Journal of Nutrition., 52(1): 379-388, 2013. M. Ozgur1, T. Ozcan, A. Akpinar-Bayizit and L. Yilmaz-Ersan. African Journal of Agricultural Research., 6 (25): 5638-5644, 2011. R. Chandini, V. Udayabhaskaran, J.P. Binoy and K.P. Ramamoorthy. International Journal of Diabetes in Developing Countries, 33(2): 83–85, 2013.

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Details

Primary Language	English
Subjects	Engineering
Journal Section	Articles
Authors	Zena A.m. Aljawadı
Publication Date	November 24, 2019
Published in Issue	Year 2019 Volume: 7

Cite

APA	Aljawadı, Z. A. (2019). Isolation and Studying the Effect of Protein Fractions of Red chili pepper (Capsicum annuum L.) on Some Biochemical Parameters in Experimental Diabetic Rats. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 7, 289-295.