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Lignocellulosic Ezymes of Basidial Fungi-Isolated from Different Ecological Niches of Georgia

Year 2021, Volume: 12 , 113 - 118, 31.12.2021

Nino Tsıklaurı Maya Jobava Rusudan Khvedelıdze Lali Kutateladze Izolda Khokhrashvılı Besik Kharatıshvılı

https://doi.org/10.55549/epstem.1038392

Abstract

Great interest in basidiomycetes for targeted technological treatment of agro-industrial plant substrates are conditioned by their ability to produce lignocellulosic enzymes. Consisting of 29 soil-climatic zones, Georgia (South Caucasus area) represents a unique place for the isolation of microorganisms. In order to study the biochemistry and physiology of wood-degrading basidiomycetes, samples of wood-degrading basidiomycetes were collected from different taxonomic niches of Georgia. 45 strains were obtained as pure cultures and 32 ones – identified. Producers of lignocellulosic enzymes were revealed among test fungi under solid-state and submerged cultivation conditions. Pleurous ostreatus GV10, Pleurotus drynus IN 11 and Fomes fomentarius GK33 were found to be the best producers of cellulosic enzymes using orange and mandarin peels, wheat and Potato straw, wheat bran as substrates, and Ganoderma lucidum GB03 – the best producer of laccase during cultivation on orange and mandarin peels waste. The influence of lignocellulose on the accumulation of the enzymes laccase, xylanase and Filter paper assay was studied.

Keywords

lignocellulosic materials Basidiomycetes laccase cellulase xylanase.

References

  • Brijwani, K., Oberoi, H. S., & Vadlani, P. V. (2010). Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran. Process Biochemistry, 45(1), 120-128.
  • Bailey, M. J., Biely, P., & Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270.
  • Bourbonnais, R., & Paice, M. G. (1990). Oxidation of non-phenolic substrates: an expanded role for laccase in lignin biodegradation. FEBS letters, 267(1), 99-102.
  • Dey, P., Rangarajan, V., Singh, J., Nayak, J., & Dilip, K. J. (2021). Current perspective on improved fermentative production and purification of fungal cellulases for successful biorefinery applications: a brief review. Biomass Conversion and Biorefinery, 1-29.
  • Fang, Z., Liu, X., Chen, L., Shen, Y., Zhang, X., Fang, W., ... & Xiao, Y. (2015). Identification of a laccase Glac15 from Ganoderma lucidum 77002 and its application in bioethanol production. Biotechnology for Biofuels, 8(1), 1-12.
  • Ghose T. (1987), Measurement of cellulose activities. Pure Appl Chem 59, 257–268.
  • Huang H., Zeng G., Tang L.,Yan H.,Yu, X. Xi, Chen Zh., Huang G. (2008), Internatiaial Biodelerioration Biodegradation 61, 331-336.
  • Heinzkill, M., Bech, L., Halkier, T., Schneider, P., & Anke, T. (1998). Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae). Applied and Environmental Microbiology, 64(5), 1601-1606.
  • Levin L, Herrmann C, Papinutti V. (2008) Biochem Eng J.39, 207–214.
  • Mahesh M., Mohini M., (2013), National Dairy Research Institute (Deemed University), Karnal, Haryana-132001.
  • Rosales, E., Couto, S. R., & Sanromán, M. A. (2007). Increased laccase production by Trametes hirsuta grown on ground orange peelings. Enzyme and Microbial Technology, 40(5), 1286-1290.
  • Thurston, C. F. (1994). The structure and function of fungal laccases. Microbiology, 140(1), 19-26.
  • Tsiklauri N., Khvedelidze R., Zakariashvili N., Aleksidze T., Bakradze-Guruli M., Kvesitadze E. (2014), Bulletin Georg. Natl. Acad. Sci., 8,102-109.
  • Yennamalli, R. M., Rader, A. J., Kenny, A. J., Wolt, J. D., & Sen, T. Z. (2013). Endoglucanases: insights into thermostability for biofuel applications. Biotechnology for Biofuels, 6(1), 1-9.

Year 2021, Volume: 12 , 113 - 118, 31.12.2021

Nino Tsıklaurı Maya Jobava Rusudan Khvedelıdze Lali Kutateladze Izolda Khokhrashvılı Besik Kharatıshvılı

https://doi.org/10.55549/epstem.1038392

Abstract

References

  • Brijwani, K., Oberoi, H. S., & Vadlani, P. V. (2010). Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran. Process Biochemistry, 45(1), 120-128.
  • Bailey, M. J., Biely, P., & Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270.
  • Bourbonnais, R., & Paice, M. G. (1990). Oxidation of non-phenolic substrates: an expanded role for laccase in lignin biodegradation. FEBS letters, 267(1), 99-102.
  • Dey, P., Rangarajan, V., Singh, J., Nayak, J., & Dilip, K. J. (2021). Current perspective on improved fermentative production and purification of fungal cellulases for successful biorefinery applications: a brief review. Biomass Conversion and Biorefinery, 1-29.
  • Fang, Z., Liu, X., Chen, L., Shen, Y., Zhang, X., Fang, W., ... & Xiao, Y. (2015). Identification of a laccase Glac15 from Ganoderma lucidum 77002 and its application in bioethanol production. Biotechnology for Biofuels, 8(1), 1-12.
  • Ghose T. (1987), Measurement of cellulose activities. Pure Appl Chem 59, 257–268.
  • Huang H., Zeng G., Tang L.,Yan H.,Yu, X. Xi, Chen Zh., Huang G. (2008), Internatiaial Biodelerioration Biodegradation 61, 331-336.
  • Heinzkill, M., Bech, L., Halkier, T., Schneider, P., & Anke, T. (1998). Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae). Applied and Environmental Microbiology, 64(5), 1601-1606.
  • Levin L, Herrmann C, Papinutti V. (2008) Biochem Eng J.39, 207–214.
  • Mahesh M., Mohini M., (2013), National Dairy Research Institute (Deemed University), Karnal, Haryana-132001.
  • Rosales, E., Couto, S. R., & Sanromán, M. A. (2007). Increased laccase production by Trametes hirsuta grown on ground orange peelings. Enzyme and Microbial Technology, 40(5), 1286-1290.
  • Thurston, C. F. (1994). The structure and function of fungal laccases. Microbiology, 140(1), 19-26.
  • Tsiklauri N., Khvedelidze R., Zakariashvili N., Aleksidze T., Bakradze-Guruli M., Kvesitadze E. (2014), Bulletin Georg. Natl. Acad. Sci., 8,102-109.
  • Yennamalli, R. M., Rader, A. J., Kenny, A. J., Wolt, J. D., & Sen, T. Z. (2013). Endoglucanases: insights into thermostability for biofuel applications. Biotechnology for Biofuels, 6(1), 1-9.
There are 14 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Nino Tsıklaurı

Maya Jobava

Rusudan Khvedelıdze

Lali Kutateladze

Izolda Khokhrashvılı

Besik Kharatıshvılı

Early Pub Date September 5, 2021
Publication Date December 31, 2021
Published in Issue Year 2021 Volume: 12

Cite

APA Tsıklaurı, N., Jobava, M., Khvedelıdze, R., … Kutateladze, L. (2021). Lignocellulosic Ezymes of Basidial Fungi-Isolated from Different Ecological Niches of Georgia. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 12, 113-118. https://doi.org/10.55549/epstem.1038392
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