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Extraction and Purification of the Potential Allergen Proteins from Botryotinia Fuckeliana

Year 2021, Volume: 12 , 28 - 31, 31.12.2021

Mustafa Koroglu Ibrahim Halil Kılıc Isik Didem Karagoz Mehmet Ozaslan

https://doi.org/10.55549/epstem.992476

Abstract

An allergy is a disease in which the immune system makes an inflammatory response to a harmless antigen. Any antigen that causes an allergy is called an allergen. Allergens may be inhaled or ingested, or they may come into contact with the skin. According to the data of the World Allergy Organization (WAO), the prevalence of allergies in different countries varies between 10-40%. Pollen, mold, animal hair, house dust mite, medicines, and foods are the most common allergen agents. Common mushrooms in nature have the potential to produce allergenic proteins. Penicillium,Botryotinia, Aspergillus, Rhizopus, and Mucor species, which are allergic fungi, are widely found in nature. In recent years, the cases of allergies caused by molds have increased significantly and studies to determine the causing allergens have accelerated. Botryotinia fuckeliana Pers. is a cosmopolitan necrotrophic pathogen infecting more than 200 plant species in temperate and subtropical climates, some of which are of high economic importance (e.g., grapes, strawberries, solanaceous vegetable). In Europe, maximal spore counts occur during late summer. Prevalence of skin test reactivity, i.e., immediate wheal and flare, to B. cinerea in Europe is comparable to that of Aspergillus spp. and greater than that of Cladosporium and Penicillium spp. Since the prevalence of B. fuckeliana spores and skin test reactivity is significant, further characterization of these allergens is warranted. Botryotinia fuckeliana was used in our study. Botryotinia fuckeliana produced in our laboratory was collected and allergen fungus protein was extracted by 2 different extraction methods. By preparing protein samples from prepared mushroom extracts, the total concentration of potential allergen proteins was determined by the BCA method. According to the data obtained, it was determined that the protein concentration of the mushroom samples dried by that were subjected to dialysis was higher than ethanol.

Keywords

Allergy Botryotinia fuckeliana Allergen protein BCA

References

  • Çeter, T. Y., & Pinar, N. M. (2008). The concentration of atmospheric pollen and spores of Kastamonu province (Central Region) and effects of meteorological factors on this concentration (January 2006- December 2007) (Doctoral dissertation, Ankara University Graduate School of Natural and Applied Sciences Department of Biology.
  • Esch, R. E., & Codina, R. (2017). Fungal raw materials used to produce allergen extracts. Annals of allergy, asthma & immunology : Official publication of the American College of Allergy, Asthma, & Immunology, 118(4), 399–405. https://doi.org/10.1016/j.anai.2016.05.012
  • Güneser, S., Atici, A., Köksal, F., & Yaman, A. (1994). Mold allergy in Adana, Turkey. Allergologia et immunopathologia, 22(2), 52–54.
  • Kendrick, B. (2000). The fifth kingdom (pp. 227-247). Hackett Publishing
  • Pawankar, R., Canonica, G. W., Holgate, S. T., Lockey, R. F., & Blaiss, M. S. (2011). WAO white book on allergy (pp. 102-110). Milwaukee, WI: World Allergy Organization, 3, 156-157.
  • Plesken, C., Weber, R. W. S., Rupp, S., Leroch, M., & Hahn, M. (2015a). Botrytis pseudocinerea is a significant pathogen of several crop plants but susceptible to displacement by fungicide-resistant B. cinerea strains. Appl. Environ. Microbiol. 81, 7048–7056. doi: 10.1128/AEM.01719-15
  • Lugauskas, A., Krikštaponis, A., & Šeškauskas, V. (2003). Species of conditionally pathogenic micromycetes in the air of dwellings and occupational premises. Indoor and Built Environment, 12(3), 167-177.
  • Simon-Nobbe, B., Denk, U., Pöll, V., Rid, R., & Breitenbach, M. (2008). The spectrum of fungal allergy. International archives of allergy and immunology, 145(1), 58–86. https://doi.org/10.1159/000107578
  • Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Analyt. Biochem. 150, 76–85.
  • Şimşekli, Y. (1994). The fungus exist in the air outside the hauses in the different regions of Bursa. (Master's thesis, Uludağ University.
  • Tao, A., & Raz, E. (Eds.). (2015). Allergy bioinformatics (8nd ed., pp.1-14). Springer.
  • Walker, J. M. (2002). The Protein Protocols Handbook (pp. 11-14). Second edition. Humana Press.
  • Wójcicka, A. (2014). Changes in pigment content of triticale genotypes infested with grain aphid Sitobion avenae (Fabricius)(Homoptera: Aphididae). Acta Biologica Cracoviensia. Series Botanica, 56(1).

Year 2021, Volume: 12 , 28 - 31, 31.12.2021

Mustafa Koroglu Ibrahim Halil Kılıc Isik Didem Karagoz Mehmet Ozaslan

https://doi.org/10.55549/epstem.992476

Abstract

References

  • Çeter, T. Y., & Pinar, N. M. (2008). The concentration of atmospheric pollen and spores of Kastamonu province (Central Region) and effects of meteorological factors on this concentration (January 2006- December 2007) (Doctoral dissertation, Ankara University Graduate School of Natural and Applied Sciences Department of Biology.
  • Esch, R. E., & Codina, R. (2017). Fungal raw materials used to produce allergen extracts. Annals of allergy, asthma & immunology : Official publication of the American College of Allergy, Asthma, & Immunology, 118(4), 399–405. https://doi.org/10.1016/j.anai.2016.05.012
  • Güneser, S., Atici, A., Köksal, F., & Yaman, A. (1994). Mold allergy in Adana, Turkey. Allergologia et immunopathologia, 22(2), 52–54.
  • Kendrick, B. (2000). The fifth kingdom (pp. 227-247). Hackett Publishing
  • Pawankar, R., Canonica, G. W., Holgate, S. T., Lockey, R. F., & Blaiss, M. S. (2011). WAO white book on allergy (pp. 102-110). Milwaukee, WI: World Allergy Organization, 3, 156-157.
  • Plesken, C., Weber, R. W. S., Rupp, S., Leroch, M., & Hahn, M. (2015a). Botrytis pseudocinerea is a significant pathogen of several crop plants but susceptible to displacement by fungicide-resistant B. cinerea strains. Appl. Environ. Microbiol. 81, 7048–7056. doi: 10.1128/AEM.01719-15
  • Lugauskas, A., Krikštaponis, A., & Šeškauskas, V. (2003). Species of conditionally pathogenic micromycetes in the air of dwellings and occupational premises. Indoor and Built Environment, 12(3), 167-177.
  • Simon-Nobbe, B., Denk, U., Pöll, V., Rid, R., & Breitenbach, M. (2008). The spectrum of fungal allergy. International archives of allergy and immunology, 145(1), 58–86. https://doi.org/10.1159/000107578
  • Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Analyt. Biochem. 150, 76–85.
  • Şimşekli, Y. (1994). The fungus exist in the air outside the hauses in the different regions of Bursa. (Master's thesis, Uludağ University.
  • Tao, A., & Raz, E. (Eds.). (2015). Allergy bioinformatics (8nd ed., pp.1-14). Springer.
  • Walker, J. M. (2002). The Protein Protocols Handbook (pp. 11-14). Second edition. Humana Press.
  • Wójcicka, A. (2014). Changes in pigment content of triticale genotypes infested with grain aphid Sitobion avenae (Fabricius)(Homoptera: Aphididae). Acta Biologica Cracoviensia. Series Botanica, 56(1).
There are 13 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Mustafa Koroglu

Ibrahim Halil Kılıc

Isik Didem Karagoz

Mehmet Ozaslan

Early Pub Date September 5, 2021
Publication Date December 31, 2021
Published in Issue Year 2021 Volume: 12

Cite

APA Koroglu, M., Kılıc, I. H., Karagoz, I. D., Ozaslan, M. (2021). Extraction and Purification of the Potential Allergen Proteins from Botryotinia Fuckeliana. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 12, 28-31. https://doi.org/10.55549/epstem.992476
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