Determination of Morphology and Allergenic Proteins of Pistachio (Pistachia vera L.) Pollens in Gaziantep
Abstract
Allergens can be defined as antigens that stimulate
specific IgE (Immunoglobulin E) antibody formation and react with it. In this
study, the morphologies of Pistachia vera
allergenic pollens belonging to Anacardiaceae family in South Eastern Anatolia
were examined by light microscopy and pollens extracts were prepared from these
species. The specimens used in morphological studies were obtained from
Gaziantep University campus area. Slides of pollens were prepared according to
Wodehouse (1935) method and the morphological characteristics of pollens were
determined using microphotographs taken by light microscopy. Pollens crude
extracts were obtaıned from the flora grown in this region. Extraction method
established by Aytug et al. (1991) was used to prepare crude extracts from the
collected pollens, 'Coca' solution was added extensively and sterile filtration
technique was used for sterilization. BCA assay and absorbance measurements
were used to determine the protein concentration. Standard curve was generated
using concentrations and absorbance values of standard protein samples prepared
at different concentrations (0, 1-1mg/ml). The protein concentration of the
pollens extracts was calculated using the correct equation obtained from this
curve. These crude extracts contain many major and minor allergens that can be
used to diagnose allergic diseases. Due to the very high concentration of
allergenic proteins in P. vera, it
can be included in allergen kits. Moreover, allergy tests should be conducted
to those living in this region. The results obtained from this study can both
contribute to systematic studies and to the treatment of allergic diseases
caused by these types of pollens.
References
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Abstract
References
- Sapan N. Polenlerin alerjik hastalıklardaki önemi. Türkiye Klinikleri J Allergy-Special Topics 2011; 4(1): 1-4. Peebles RS, Church M and Durham SR. Principles of allergy diagnosis. Holgate S, Church M, Broide D, and Martinez F, ed. Allergy. 4th Ed. Elsevier 2012;129 Behrendt H, Becker WF. Localization, release and bioavailability of pollen allergens: The influence of environmental factors. Current Opinion in Immunology 2001;13:709-15. Bıçakçı, A., & Tosunoğlu, A. (2017). The Influence of Environmental and Atmospheric Variables on Allergenic Pollen. Asthma Allergy Immunology, 14(3), 107-116. Spieksma, F.T.M., 1991, Regional European pollen calendars. In: D’Amato G, Spieksma, F.T.M, Bonini S editors, Allergenic pollen and pollinosis in Europe. Oxford: Blackwell Sci. Publ., 49–65. Ventura, M.T, Gelardi, M., Di Gioia, R., Buquicchio, R., Accettura, G., Tummolo, R. A. and et al., 2007, Statistical evaluation and parameters ofphlogosis in patients sensitized to cypress, Journal of biological regulators and homeostatic agents, 21:41-8. Movérare, R., Everberg, H., Carlsson, R., Holtz, A., Thunberg, R., Olsson, P., ... & Högbom, E. (2008). Purification and characterization of the major oak pollen allergen Que a 1 for component-resolved diagnostics using ImmunoCAP®.International archives of allergy and immunology, 146(3), 203-211. Aytuğ B, Peremeci E. (1987). Pollen grains; pollinosis and pollen extracts. Journal of Faculty of Medicine I.U, 50, 163-170. Wodehouse R.P. (1935). Pollen Grains. New York: McGraw-Hill.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | August 19, 2018 |
| Published in Issue | Year 2018Issue: 2 |
