Determination of Potential Allergenic Proteins and Morphologenic Proteins and Morphology of Linden (Tilia cordata), Anatolian oak (Quercus ithaburensis) and Birch (Betula alba) Pollens in Gaziantep
Abstract
Allergic diseases are a major public health problem
in the modern societies. Pollens dispersed through wind are one of the major aeroallergens.
When they are released to atmosphere in sufficient amount, they can cause the
development of diseases such as asthma, rhinitis, rhinoconjunctivitis etc. in
allergically hypersensitive individuals. The spreading of pollens are
influenced by their amount in the air, their structures, geographic areas and
the climate. Therefore, the types of pollens that hypersensitive individuals
who live in different regions are exposed to may differ and different allergic
reactions may occur in affected individuals. The high amount of allergens in
pollens enhance the sensitivity to pollens. Thus the research in
region-specific plant species allergenic effects is very important. Pollens of
linden (Tilia cordata), Anatolian oak
(Quercus ithaburensis) and birch (Betula alba) are important allergen
sources in Gaziantep province. Pollen allergens are water soluble, stable
proteins or glycoproteins of molecular weight between 5-80 kD. A single pollen
type usually contains several different allergens. Pollens from linden (T. cordata), Anatolian oak (Q. ithaburensis) and birch (B. alba) were collected during
pollination period and their extracts were prepared. For identification of
pollens morphology slides were prepared according to Wodehouse’s method and
images were taken under light microscope. Total concentrations of potential
allergen proteins were determined from prepared pollen extracts using the BCA
method. In this study, we aimed to prepare extracts of pollens from linden,
Anatolian oak and birch widely grown in wooded areas of Gaziantep University
for the study of allergens and their use for diagnosing allergic diseases.
References
- Behrendt, H., & Becker, W. M. (2001). Localization, release and bioavailability of pollen allergens: the influence of environmental factors. Current opinion in immunology, 13(6), 709-715. Cacciola, R. R., Sarva, M., & Polosa, R. (2002). Adverse respiratory effects and allergic susceptibility in relation to particulate air pollution: flirting with disaster. Allergy, 57(4), 281-286. D'amato, G. (2002). Environmental urban factors (air pollution and allergens) and the rising trends in allergic respiratory diseases. Allergy, 57(s72), 30-33. Çelenk, S. (2011). Bazı Polenler Neden Daha Allerjik?. Turkiye Klinikleri Journal of Immunology Allergy Special Topics, 4(1), 5-9. Heinzerling, L., Mari, A., Bergmann, K. C., Bresciani, M., Burbach, G., Darsow, U. & Bom, A. T. (2013). The skin prick test–European standards. Clinical and translational allergy, 3(1), 3. Movérare, R., Everberg, H., Carlsson, R., Holtz, A., Thunberg, R., Olsson, P., ... & Högbom, E. (2008). Purification and characterization of the major oak pollen allergen Que a 1 for component-resolved diagnostics using ImmunoCAP®.International archives of allergy and immunology, 146(3), 203-211. Wodehouse R.P. (1935). Pollen Grains. New York: McGraw-Hill. Aytuğ B. 1996. Polen Ekstrelerinin Hazırlanması. Yüksek Lisans Ders Notları. Walker J M. 2002. The Protein Protocols Handbook. Second edition. Humana Press.
Abstract
References
- Behrendt, H., & Becker, W. M. (2001). Localization, release and bioavailability of pollen allergens: the influence of environmental factors. Current opinion in immunology, 13(6), 709-715. Cacciola, R. R., Sarva, M., & Polosa, R. (2002). Adverse respiratory effects and allergic susceptibility in relation to particulate air pollution: flirting with disaster. Allergy, 57(4), 281-286. D'amato, G. (2002). Environmental urban factors (air pollution and allergens) and the rising trends in allergic respiratory diseases. Allergy, 57(s72), 30-33. Çelenk, S. (2011). Bazı Polenler Neden Daha Allerjik?. Turkiye Klinikleri Journal of Immunology Allergy Special Topics, 4(1), 5-9. Heinzerling, L., Mari, A., Bergmann, K. C., Bresciani, M., Burbach, G., Darsow, U. & Bom, A. T. (2013). The skin prick test–European standards. Clinical and translational allergy, 3(1), 3. Movérare, R., Everberg, H., Carlsson, R., Holtz, A., Thunberg, R., Olsson, P., ... & Högbom, E. (2008). Purification and characterization of the major oak pollen allergen Que a 1 for component-resolved diagnostics using ImmunoCAP®.International archives of allergy and immunology, 146(3), 203-211. Wodehouse R.P. (1935). Pollen Grains. New York: McGraw-Hill. Aytuğ B. 1996. Polen Ekstrelerinin Hazırlanması. Yüksek Lisans Ders Notları. Walker J M. 2002. The Protein Protocols Handbook. Second edition. Humana Press.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | August 19, 2018 |
| Published in Issue | Year 2018Issue: 2 |
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