Different substances are used to either eliminate or decrease the
numbers of rumen bacteria to alter their makeup. Essential oils (EO) are one of
the substances used for this purpose. The present study was carried out to
determine the effects of EO extracted from orange peel (Citrus cinensis),
cinnamon (Cinnamomumverum), Laurel (Laurusnobilis), oleaster
(Eleagnusangustifolia), garlic (Allium sativum) and thyme (Tymusvulgare) on
Streptococcus bovis (ES1). For this purpose, bacterial growth was measured by
inoculating stock cultures grown in Hobson's M8 medium with a three-fold
increasing series of EO. Essential oil diluted in autoclaved water containing
10% DMSO was added aseptically after the medium was autoclaved to give final
concentrations ranging from 50 to 5,000 ppm (0.5 ml to each 6.5 ml of M8).
Bacterial growth was measured by reading the optical density at 650 nm hourly
until the reading for bacterial growth decreased. Maximal bacterial growth rate
was calculated using the MicroFit v 1.0. The results show that the effects of
essential oils, doses and dose-oil interactions used in the study are
statistically significant. According to the results, garlic and cinnamon
essential oil have strong antimicrobial activity on Streptococcus bovis (ES1).
Primary Language | English |
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Journal Section | Articles |
Authors | |
Publication Date | December 1, 2018 |
Published in Issue | Year 2018Issue: 3 |