Research Article
Year 2018,
Issue: 4, 224 - 229, 04.12.2018
Abstract
Hearth
is an important organ that is a responsible for pumping blood whole body, and
so cardiac diseases are critically important all over the world. Pericardial fluid (PF) and its component are
important for diagnosis and treatment for heart disease. In literature, PF has
been analysed especially for pericarditis disease, and there has been any
research on PF for tissue engineering application. The aim of this study is
isolating the cells from the pericardial fluid and culturing these cells in vitro
in order to be used as cell line in tissue engineering studies. PF contains
heterogen cell population which are lymphocytes, glanulocytes, macrophages,
eosinophils and basophils, These cells were cultured in five different media
(Alpha MEM, DMEM-Low, EMEM, MCDB 131, and Med 199) in this study. As
a result of cell culture was performed and concluded that, cell proliferation
and viability were the best in culture with Alpha MEM media according to the microscope examination.
References
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Year 2018,
Issue: 4, 224 - 229, 04.12.2018
Abstract
References
- Athar H, Parrah JD, Moulvi BA, Singh M, Dedmari FH (2012) “Pericarditis in Bovines: A Review” . International Journal of Advanced Veterinary Science and Technology. 1 (1): 19-27. Benhaiem-Sigaux, N., Mina, E., Sigaux, F., Lambre, C. R., Valensi, F., Allegret, C., et al. (1985). “Characterization of human pericardial macrophages”, J. Leukoc. Biol. 38, 709–721 Blázquez R, Sánchez-Margallo FM, Crisóstomo V, Báez C, Maestre J, García-Lindo M, et al. (2015), “Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis” PLoS ONE 10(3): e0122377. DOI:10.1371/journal.pone.0122377 Chinchoy, E., Ujhelyi, M. R., Hill, A. J., Skadsberg, N. D., & Iaizzo, P. A. (2005) “The pericardium”, In Handbook of Cardiac Anatomy, Physiology, and Devices (pp. 101-110). Humana Press. DOI: 10.1007/978-1-59259-835-9_7 Gibson, A., T., and Segal, M., B., (1978a). “A study of the composition of pericardial fluid, with special reference to the probable mechanism of fluid formation” J. Physiol. 277, 367–377. doi: 10.1113/jphysiol.1978.sp012277 Gibson, A. T., and Segal, M. B. (1978b). “A study of the routes by which protein passes from the pericardial cavity to the blood in rabbits”, J. Physiol. 280, 423–433. Holt, J., P., (1970). “The normal pericardium” Am. J. Cardiol. 26, 455–465. doi: 10.1016/0002-9149(70)90702-2 Iskandar R., Liu S., Xiang F.,and et al. (2017) “Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure”, Experimental and Therapeutic Medicine. 2017, Vol.13, No.5, p.1850. Pekins S.L., et al. (2004) “Pericarditis and Pleuritis Caused By C. Pseudotuberculosis in a Horse”, J AmVet Med Assoc.. 224; 1133-1138. Vogiatzidis K., Zarogiannis S.G., Aidonidis I., et al. (2015) “Physiology of pericardial fluid production and drainage”, Front Physiol.;18:6–62. doi: 10.3389/fphys.2015.00062
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Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 4, 2018 |
| Published in Issue | Year 2018Issue: 4 |
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