Research Article
Year 2019,
Volume: 6 , 88 - 95, 25.07.2019
Abstract
References
- Ajjawi, I., Verruto, J., Aqui, M., Soriaga, L. B., Coppersmith, J., & Kwok, K., et al. (2017, Jun 19). Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator. National Biotechnology. 35, 647–652. Balat, M., & Balat, H. (2009, May19). Biogas as a renewable energy source – a review. Journal of Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 31, 1280-1293. Bligh, E.G., & Dyer, W.J. (1959, August). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8):911-917. Brennan, L., & Owende, P. (2010). Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products. Renewable and Sustainable Energy Review, 14,557–577. Chaturvedi, R., & Fujita, Y. (2006, September 14). Isolation of enhanced eicosapentaenoic acid producing mutants of Nannochloropsis oculata ST-6 using ethyl methane sulfonate induced mutagenesis techniques and their characterization at mRNA transcript level. Phycological Research, 54(3),208- 219. Chien-Ya, K., Sheng-Yi, C., Huang, T., Dai, L., Hsu, L., & Lin, C. (2012, January 24). Ability of a mutant strain of the microalgae Chlorella sp. to capture carbon dioxide for biogas upgrading. Journal of Applied Energy 93,176-183. ELSEVIER. Doan, T.T.Y., & Obbard, J..P. (2012). Enhanced intracellular lipid in Nannochloropsis sp. via random mutagenesis and flow cytometric cell sorting. Algal Research 1, 17–21. Jabeen, N., & Mirza, B. (2004). Ethyl methane sulphonate induces morphological mutations in Caspicum annuum. International Journal of Agriculture and Biology, 6 (2), 340-345. Kassim, T., Al-Saadi, H., & Salman, N. (1999). Production of some phyto-and zooplankton and their use as live food for fish larva.Iraqi Journal of Agriculture and Processing,. 2,188-201. Kawaroe, M.., Agus, O.S., Junkwan, H., & Dina, A. (2015). Chemical mutagenesis of microalgae Nannochloropsis sp. using EMS (ethyl Methanesulfonate). British Journal of Applied Science & Technology 8(5), 494-505. Kim,.Y., Schumaker, K..S., & Zhu, J..K. (2006). EMS mutagenesis of Arabidopsis methods. Mol. Biol., 323( 6), 101–3. Lee, B., Choi, G.G., Choi, Y.E., Sung, M., Park, M. S., & Yang, J .W. (2014, June). Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii. Korean Journal of Chemical Engineering, 31(6), 1036–1042. Mackinney G (1941, March 13). Absorption of light by chlorophyll solutions. Journal of biological Chemistry 140 (2):315-322. Mendoza, H., de la Jara, A., Freijanes, K., Carmona, L., Ramos, A.A., de Sousa Duarte, V., & Serafim Varela, J.C. (2008, October 15). Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains. Electronic Journal of Biotechnology, 11(4), 1-13. Mobini-Dehkordi, M., Nahvi, I., Zarkesh-Esfahani, H., Ghaedi, K.., Tavassoli, M., & Akada, R. (2008). Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanol. Journal of Bioscience and Bioengineering, 105(4), 403–408. Muto, M., Fukuda, Y., Nemoto, M., Yoshino, T., Matsunaga, T., &Tanaka, T. (2013, February). Establishment of a genetic transformation system for the marine pennate diatom Fistulifera sp. strain JPCC DA0580-a high triglyceride producer. Mar. Biotechnology. 15(1), 48–55. Olofsson, M., Lamela, T., Nilsson, E., Bergé, J.P., Pino, V., Uronen, P., & Legrand, C. (2012, May 21). Seasonal variation of lipids and fatty acids of the microalgae Nannochloropsis oculata grown in outdoor large-scale photobioreactors. Energies, 5, 1577-1592. Ong, S.C., Kao, C.Y., Chiu, S.Y., Tsai, M.T., & Lin, C.S. (2010, April). Characterization of the thermaltolerant mutants of Chlorella sp. with high growth rate and application in outdoor photobioreactor cultivation. Bioresearch Technology 101, 2880–2883. Polle, J.E., Kanakagiri, S., Jin, E., Masuda, T., & Melis, A. (2002, December). Truncated chlorophyll antenna size of the photosystems—A practical method to improve microalgal productivity and hydrogen production in mass culture. International Journal of Hydrogen. Energy 27(11-12), 1257–1264. Radakovits, R., Jinkerson, R.E., Darzins, A., & Posewitz, M.C. (2010, April). Genetic engineering of algae for enhanced biofuel production. Eukaryot. Cell, 9(40), 486–501. Rasha, K..M., & Kadium, M. I. (2017, March). Cytological effect of mutagenic agents and NaCl on mitotic division in two Iraqi rice (Oryza sativa L.) genotypes. Journal of Al-Nahrain University 20 (1), 116-122. Raunkjær, K., Hvitved-Jacobsen, T., & Nielsen, P.H. (1994, February). Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water research. 28(2), 251-262. Sahu, A., Pancha, I., Jain, D., Paliwal, C., Ghosh, T., Patidar, S., Bhattacharya, S., & Mishra, S. (2013, February28). Fatty acids as biomarkers of microalgae. Phytochemistry. 89:53-8. Sandesh, B., Kamath, R., Vidhyavathi, R. S., & Ravishankar, G.A. (2008, December). Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants. Bioresource Technology, 99(18), 8667 –8673. Shin, W.S., Lee, B., Jeong, B.R., Chang, Y.K., & Kwon, J.H. (2016). Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity. Journal of Applied Phycology 28, 3193–3202. Wei, H., Shi, Y., Ma, X., Pan, Y., Hu, H., Li, Y. et al. (2017, July). A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga, Nannochloropsis oceanica. Biotechnology Biofuels 10:174. Work, V.H., Radakovits, R., Jinkerson, R.E., Meuser, J.E., Elliott, L.G., Vinyard, D.J., Laurens, L.M.L., Dismukes, G.C., & Posewitz, M.C. (2010, August). Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains. Eukaryot Cell 9 (8), 1251–61. Zienkiewicz, K., Zienkiewicz, A., Poliner, E., Du, Z. Y., Vollheyde, K., Herrfurth, C., et al. (2017, January 3). Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts. Biotechnology for Biofuels10:8.
Year 2019,
Volume: 6 , 88 - 95, 25.07.2019
Abstract
Mutant microalgae Nanochloropsis oculata. and Chlorella sp
included in this study exhibited a high potential for energetic lipid storage
as well as high growth rates comparing to the un mutants (standard), therefore,
considered promising candidates for advancing the biofuel production. In
addition to the exponentially increase in biomass carbohydrates, proteins and
fatty acids remarkably increased after these strains were mutated by ethyl
methane sulphonate (EMS), while total lipids decreased. Biochemical
compositional analysis was carried out by using gas chromatography to find out
concentrations of five major fatty acids: Palmitic acid, Lenolenic acid,
Stearic acid, Oleic acid and Linoleic acid. In mutant N. oculata and Chlorella
sp. Lenolenic acid is the fatty acid
with high percentage (11.7 and 4.8 %) respectively compared with un- mutant (0.241
and 1.824) %. Five fatty acids were selected to assess effect of the mutation,
which revealed that initial concentrations of all the fatty acids before the
mutation were multiplied about 48, 16, 14, 7 and 6 fold of Lenolenic, Palmitic,
Oleic, Stearic and Linoleic acids respectively in N. oculata, while slightly
changes occurred in the concentrations of Chlorella sp fatty acids.
Keywords
References
- Ajjawi, I., Verruto, J., Aqui, M., Soriaga, L. B., Coppersmith, J., & Kwok, K., et al. (2017, Jun 19). Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator. National Biotechnology. 35, 647–652. Balat, M., & Balat, H. (2009, May19). Biogas as a renewable energy source – a review. Journal of Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 31, 1280-1293. Bligh, E.G., & Dyer, W.J. (1959, August). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8):911-917. Brennan, L., & Owende, P. (2010). Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products. Renewable and Sustainable Energy Review, 14,557–577. Chaturvedi, R., & Fujita, Y. (2006, September 14). Isolation of enhanced eicosapentaenoic acid producing mutants of Nannochloropsis oculata ST-6 using ethyl methane sulfonate induced mutagenesis techniques and their characterization at mRNA transcript level. Phycological Research, 54(3),208- 219. Chien-Ya, K., Sheng-Yi, C., Huang, T., Dai, L., Hsu, L., & Lin, C. (2012, January 24). Ability of a mutant strain of the microalgae Chlorella sp. to capture carbon dioxide for biogas upgrading. Journal of Applied Energy 93,176-183. ELSEVIER. Doan, T.T.Y., & Obbard, J..P. (2012). Enhanced intracellular lipid in Nannochloropsis sp. via random mutagenesis and flow cytometric cell sorting. Algal Research 1, 17–21. Jabeen, N., & Mirza, B. (2004). Ethyl methane sulphonate induces morphological mutations in Caspicum annuum. International Journal of Agriculture and Biology, 6 (2), 340-345. Kassim, T., Al-Saadi, H., & Salman, N. (1999). Production of some phyto-and zooplankton and their use as live food for fish larva.Iraqi Journal of Agriculture and Processing,. 2,188-201. Kawaroe, M.., Agus, O.S., Junkwan, H., & Dina, A. (2015). Chemical mutagenesis of microalgae Nannochloropsis sp. using EMS (ethyl Methanesulfonate). British Journal of Applied Science & Technology 8(5), 494-505. Kim,.Y., Schumaker, K..S., & Zhu, J..K. (2006). EMS mutagenesis of Arabidopsis methods. Mol. Biol., 323( 6), 101–3. Lee, B., Choi, G.G., Choi, Y.E., Sung, M., Park, M. S., & Yang, J .W. (2014, June). Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii. Korean Journal of Chemical Engineering, 31(6), 1036–1042. Mackinney G (1941, March 13). Absorption of light by chlorophyll solutions. Journal of biological Chemistry 140 (2):315-322. Mendoza, H., de la Jara, A., Freijanes, K., Carmona, L., Ramos, A.A., de Sousa Duarte, V., & Serafim Varela, J.C. (2008, October 15). Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains. Electronic Journal of Biotechnology, 11(4), 1-13. Mobini-Dehkordi, M., Nahvi, I., Zarkesh-Esfahani, H., Ghaedi, K.., Tavassoli, M., & Akada, R. (2008). Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanol. Journal of Bioscience and Bioengineering, 105(4), 403–408. Muto, M., Fukuda, Y., Nemoto, M., Yoshino, T., Matsunaga, T., &Tanaka, T. (2013, February). Establishment of a genetic transformation system for the marine pennate diatom Fistulifera sp. strain JPCC DA0580-a high triglyceride producer. Mar. Biotechnology. 15(1), 48–55. Olofsson, M., Lamela, T., Nilsson, E., Bergé, J.P., Pino, V., Uronen, P., & Legrand, C. (2012, May 21). Seasonal variation of lipids and fatty acids of the microalgae Nannochloropsis oculata grown in outdoor large-scale photobioreactors. Energies, 5, 1577-1592. Ong, S.C., Kao, C.Y., Chiu, S.Y., Tsai, M.T., & Lin, C.S. (2010, April). Characterization of the thermaltolerant mutants of Chlorella sp. with high growth rate and application in outdoor photobioreactor cultivation. Bioresearch Technology 101, 2880–2883. Polle, J.E., Kanakagiri, S., Jin, E., Masuda, T., & Melis, A. (2002, December). Truncated chlorophyll antenna size of the photosystems—A practical method to improve microalgal productivity and hydrogen production in mass culture. International Journal of Hydrogen. Energy 27(11-12), 1257–1264. Radakovits, R., Jinkerson, R.E., Darzins, A., & Posewitz, M.C. (2010, April). Genetic engineering of algae for enhanced biofuel production. Eukaryot. Cell, 9(40), 486–501. Rasha, K..M., & Kadium, M. I. (2017, March). Cytological effect of mutagenic agents and NaCl on mitotic division in two Iraqi rice (Oryza sativa L.) genotypes. Journal of Al-Nahrain University 20 (1), 116-122. Raunkjær, K., Hvitved-Jacobsen, T., & Nielsen, P.H. (1994, February). Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water research. 28(2), 251-262. Sahu, A., Pancha, I., Jain, D., Paliwal, C., Ghosh, T., Patidar, S., Bhattacharya, S., & Mishra, S. (2013, February28). Fatty acids as biomarkers of microalgae. Phytochemistry. 89:53-8. Sandesh, B., Kamath, R., Vidhyavathi, R. S., & Ravishankar, G.A. (2008, December). Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants. Bioresource Technology, 99(18), 8667 –8673. Shin, W.S., Lee, B., Jeong, B.R., Chang, Y.K., & Kwon, J.H. (2016). Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity. Journal of Applied Phycology 28, 3193–3202. Wei, H., Shi, Y., Ma, X., Pan, Y., Hu, H., Li, Y. et al. (2017, July). A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga, Nannochloropsis oceanica. Biotechnology Biofuels 10:174. Work, V.H., Radakovits, R., Jinkerson, R.E., Meuser, J.E., Elliott, L.G., Vinyard, D.J., Laurens, L.M.L., Dismukes, G.C., & Posewitz, M.C. (2010, August). Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains. Eukaryot Cell 9 (8), 1251–61. Zienkiewicz, K., Zienkiewicz, A., Poliner, E., Du, Z. Y., Vollheyde, K., Herrfurth, C., et al. (2017, January 3). Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts. Biotechnology for Biofuels10:8.
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Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | July 25, 2019 |
| Published in Issue | Year 2019Volume: 6 |
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