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Year 2019, Volume: 7 , 296 - 307, 24.11.2019

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References

  • Albadah, M.S.; Dekhil, H.; Shaik, S.A.; Alsaif, M.A ; Shogair, M; Nawaz, S. and Alfadda, A.A. (2015). '' Effect of weight loss on serum osteocalcin and its association with serum adipokines''. Internat. J. Endocrinol. 10: 1-10. Alfadda, A.A.; Masood, A.; Shaik, S.A.; Dekhil, H. and Goran, M. (2013). ''Association between osteocalcin, metabolic syndrome, and cardiovascular risk factors: Role of total and undercarboxylated osteocalcin in patients with type 2 diabetes''. Internat. J. Endocrinol. 10: 1-6. Allain, C.C.; Poon, L.S.; Chan, C.S.; Richmond, W. and Fu, P.C. (1974). ''Determination of serum total cholesterol by enzymatic colorimetric method''. J. Clin. Chem. 20(4): 470-475. Burtis, C. A. and Ashwood, E.R. (1982). '' Teits Fundamental of Clinical Chemistry''. 2nd ed., W.B. Saunders Company, U.S.A. Philadelphia. :302, 337, 539, 901. Chabrol and Chardonnet. (1937). Cited by AL-Mollah, L. A. M. (1990). ''Studies of some metabolic effects of noradrendine insulin inpresence of Ca++. M.Sc. Thesis, University of Mosul. Dou, J.; Li, H.; Ma, X.; Zhang, M.; Fang, Q.; Nie, M.; Bao, Y. and Jia, W. (2014). ''Osteocalcin attenuates high fat diet-induced impairment of endothelium-dependent relaxation through Akt/eNOS-dependent pathway''. J. Cardiovascular Diabetol. 13(74): 1-12. Fossati, P. and Principle, L. (1982). ''Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide''. J. Clin. Chem. 28(10): 2077-2080. Grau, G.A.; Ara, I., Dorado, C.; Rodriguez, V.G.; Gomez, P.J.; Cabrero, J.C.; Sanchez, S.J.A.; Santana, A. and Calbet, J.A. (2010). ''Osteocalcin as a negative regulator of serum leptin concentration in humans: insight from triathlon competitions''. Eur. J. Appl. Physiol. 110(3): 635-643. Kang, J.H. (2016). '' Association of Serum Osteocalcin with Insulin Resistance and Coronary Atherosclerosis''. J. Bone Metab. 23: 183-190. Kirkwood, B. R. (1988). Essentials of Medical Statistics. 1st edn., Black well Scientific Publication, Oxford; 43-56. Lacombe, J.; Karsenty, G. and Ferron, M. (2013). ''In vivo analysis of the contribution of bone resorption to the control of glucose metabolism in mice''. J. Molecular. Metab. 8: 1-7. Lee, N.K.; Sowa, H.; Hinoi, e.; Ferron, M.; Ahn, J.D.; Confavreux, C.; Dacquin, R.; Mee, P.J.; Mckee, M.D.; Jung, D.Y.; Zhang, Z.; Kim, J.K.; Mauvais,J. F; Ducy, P. and Karsenty, G. (2007). ''Endocrine regulation of energy metabolism by the skeleton''. J. Cell. 130: 456-469. Miura, T.; Usami, M.; Tsuura, Y.; Ishida, H. and Seino, Y. (1995). ''Hypoglycemic and hyolipidemic effect of chitosan in normal and neonantal streptotocin induced diabetic mice''. J. Biol. Pharm. Bull. 18(1): 1623-1625. Nagasue, K.; Inaba, M.; Okuno, S.; Kitatani, K.; Imanishi, Y.; Ishimura, E.; Miki, T., Kim, M. and Nishizawa, Y. (2003). ''Serum N-terminal midfragment vs. intact osteocalcin immunoradiometric assay as markers for bone turnover and bone loss in hemodialysis patients''. J. Biomed. Pharmacother. 57(2):98-104. Nansseu, J.R.N.; Moor, V.J.A.; Nouaga, M.E.D.; Awona, B.Z.; Tchanana, G. and Ketcha, A. (2016). '' Atherogenic index of plasma and risk of cardiovascular disease among Cameroonian postmenopausal women''. J. Lipids in Health and Disease. 15:49: 1-5. O'Connor, E.M. and Durack, E. (2017). '' Osteocalcin: The extra-skeletal role of a vitamin K-dependent protein in glucose metabolism''. J. Nutr. & Intermed. Metab. 7: 8-13. Pi, M. and Quarles, L.D. (2012). ''Multiligand specificity and wide tissue expression of GPRC6A reveals new endocrine networks''. J. Endocrinol. 153(5): 2062-2069. Rehder, D.S.; Gundberg, C.M.; Booth, S.L. and Borges, C.R. (2015). '' Gamma-carboxylation and fragmentation of osteocalcin in human serum defined by mass spectrometry''. J. Mol. & Cell. Proteomics. 14(6): 1546-1555. Research shows skeleton to be endocrine organ. http://www.columbia.edu/cu. (2007). Robyt, F.J. and White, J. B. (1987). "Biochemical Techniques Theory and Practice". Books Cole Publishing Co., U.S.A., 141, 235-236, 246, 263, 269 Sabek, O.M.; Nishimoto, S.K.; Fraga, D.; Tejpal, N.; Ricordi, C. and Gaber, A.O. (2015). ''Osteocalcin effect on human β-cells mass and function''. J. Endocrinol. 156(9): 3137-3146. Saravanan, G; Kalaiselvi, .B. and Reddy, G.P.(2011). ''Hypolipidemic effect of zignidd (polyherbal formulation) in streptozotocin-induced diabetic rats''. INT. J. Drug Formulation and Research. 2(6): 151-160.20. Schacterle, G.R. and Pollack , R. L. (1973). ''A simplified method for the quantitatiue assay of small amounts of protein biological material''. J. Anal. Biochem. 51: 654-655. Shao, J.; Wang, Z.; Yang, T.; Ying, H.; Zhang, Y. and Liu, S. '' (2015). ''Bone regulates glucose metabolism as an endocrine organ through osteocalcin''. Internat. J. Endocrinol. 10: 1-9. Tomoda, M.; Shimizu, N.; Gonda, R.; Kanari, M.; Yamada, H. and Hikino, H. (1990). ''Anti-Complementary and hypoglycemic activities of the glycans from the seeds of malva verticillata''. J. Planta. Med. 56:168-170. Yeboah, J.O.; Owiredu, W.K.B.A.; Norgbe, G.K.; Lokpo, S.Y.; Gyamfi,J.; Allotey, E.A.; Aduko, R.A.; Noagbe, M. and Attah, F.A. (2017). '' The Prevalence of metabolic syndrome and its components among people with type 2 diabetes in the Ho Municipality, Ghana: A cross-sectional study''. Internat. J. Chronic Dis. 10: 1-8. Zoch, M.L.; Clemens, T.L. and Riddle, R.C. (2016). '' New insights into the biology of osteocalcin''. J. Bone and diabetes. 82: 42–49.

Study the Effect of Isolated Osteocalcin from Human and Rats on Lipids Profile in Normal and Alloxan-Induced Diabetic Male Rats

Year 2019, Volume: 7 , 296 - 307, 24.11.2019

Abstract

This study concerned with an attempts to isolate and purify osteocalcin from healthy human blood plasma and healthy rats serum using different biochemical techniques. This included precipitation by cold acetone and gel filtration chromatography. It was found that only the second peak (peak B) from human and rats had concentration of osteocalcin, and showed that fold of purification of isolated osteocalcin 57 and 43 for human and rats respectively. The research also included study the effect of isolated osteocalcin from human plasma and rats serum on lipids profile in normal and alloxan induced diabetic males rats. The results showed after one week of treatment by the isolated osteocalcin from human plasma and rats serum at the dose (1ng/Kg of body weight/d) caused a significant decrease in the concentration of all from total lipids, total cholesterol, triglycerides, VLDL-C, LDL-C, atherogenic index, and a significant increase in the concentration of HDL-C, and antiatherogenic index in normal and alloxan induced diabetic rats.

References

  • Albadah, M.S.; Dekhil, H.; Shaik, S.A.; Alsaif, M.A ; Shogair, M; Nawaz, S. and Alfadda, A.A. (2015). '' Effect of weight loss on serum osteocalcin and its association with serum adipokines''. Internat. J. Endocrinol. 10: 1-10. Alfadda, A.A.; Masood, A.; Shaik, S.A.; Dekhil, H. and Goran, M. (2013). ''Association between osteocalcin, metabolic syndrome, and cardiovascular risk factors: Role of total and undercarboxylated osteocalcin in patients with type 2 diabetes''. Internat. J. Endocrinol. 10: 1-6. Allain, C.C.; Poon, L.S.; Chan, C.S.; Richmond, W. and Fu, P.C. (1974). ''Determination of serum total cholesterol by enzymatic colorimetric method''. J. Clin. Chem. 20(4): 470-475. Burtis, C. A. and Ashwood, E.R. (1982). '' Teits Fundamental of Clinical Chemistry''. 2nd ed., W.B. Saunders Company, U.S.A. Philadelphia. :302, 337, 539, 901. Chabrol and Chardonnet. (1937). Cited by AL-Mollah, L. A. M. (1990). ''Studies of some metabolic effects of noradrendine insulin inpresence of Ca++. M.Sc. Thesis, University of Mosul. Dou, J.; Li, H.; Ma, X.; Zhang, M.; Fang, Q.; Nie, M.; Bao, Y. and Jia, W. (2014). ''Osteocalcin attenuates high fat diet-induced impairment of endothelium-dependent relaxation through Akt/eNOS-dependent pathway''. J. Cardiovascular Diabetol. 13(74): 1-12. Fossati, P. and Principle, L. (1982). ''Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide''. J. Clin. Chem. 28(10): 2077-2080. Grau, G.A.; Ara, I., Dorado, C.; Rodriguez, V.G.; Gomez, P.J.; Cabrero, J.C.; Sanchez, S.J.A.; Santana, A. and Calbet, J.A. (2010). ''Osteocalcin as a negative regulator of serum leptin concentration in humans: insight from triathlon competitions''. Eur. J. Appl. Physiol. 110(3): 635-643. Kang, J.H. (2016). '' Association of Serum Osteocalcin with Insulin Resistance and Coronary Atherosclerosis''. J. Bone Metab. 23: 183-190. Kirkwood, B. R. (1988). Essentials of Medical Statistics. 1st edn., Black well Scientific Publication, Oxford; 43-56. Lacombe, J.; Karsenty, G. and Ferron, M. (2013). ''In vivo analysis of the contribution of bone resorption to the control of glucose metabolism in mice''. J. Molecular. Metab. 8: 1-7. Lee, N.K.; Sowa, H.; Hinoi, e.; Ferron, M.; Ahn, J.D.; Confavreux, C.; Dacquin, R.; Mee, P.J.; Mckee, M.D.; Jung, D.Y.; Zhang, Z.; Kim, J.K.; Mauvais,J. F; Ducy, P. and Karsenty, G. (2007). ''Endocrine regulation of energy metabolism by the skeleton''. J. Cell. 130: 456-469. Miura, T.; Usami, M.; Tsuura, Y.; Ishida, H. and Seino, Y. (1995). ''Hypoglycemic and hyolipidemic effect of chitosan in normal and neonantal streptotocin induced diabetic mice''. J. Biol. Pharm. Bull. 18(1): 1623-1625. Nagasue, K.; Inaba, M.; Okuno, S.; Kitatani, K.; Imanishi, Y.; Ishimura, E.; Miki, T., Kim, M. and Nishizawa, Y. (2003). ''Serum N-terminal midfragment vs. intact osteocalcin immunoradiometric assay as markers for bone turnover and bone loss in hemodialysis patients''. J. Biomed. Pharmacother. 57(2):98-104. Nansseu, J.R.N.; Moor, V.J.A.; Nouaga, M.E.D.; Awona, B.Z.; Tchanana, G. and Ketcha, A. (2016). '' Atherogenic index of plasma and risk of cardiovascular disease among Cameroonian postmenopausal women''. J. Lipids in Health and Disease. 15:49: 1-5. O'Connor, E.M. and Durack, E. (2017). '' Osteocalcin: The extra-skeletal role of a vitamin K-dependent protein in glucose metabolism''. J. Nutr. & Intermed. Metab. 7: 8-13. Pi, M. and Quarles, L.D. (2012). ''Multiligand specificity and wide tissue expression of GPRC6A reveals new endocrine networks''. J. Endocrinol. 153(5): 2062-2069. Rehder, D.S.; Gundberg, C.M.; Booth, S.L. and Borges, C.R. (2015). '' Gamma-carboxylation and fragmentation of osteocalcin in human serum defined by mass spectrometry''. J. Mol. & Cell. Proteomics. 14(6): 1546-1555. Research shows skeleton to be endocrine organ. http://www.columbia.edu/cu. (2007). Robyt, F.J. and White, J. B. (1987). "Biochemical Techniques Theory and Practice". Books Cole Publishing Co., U.S.A., 141, 235-236, 246, 263, 269 Sabek, O.M.; Nishimoto, S.K.; Fraga, D.; Tejpal, N.; Ricordi, C. and Gaber, A.O. (2015). ''Osteocalcin effect on human β-cells mass and function''. J. Endocrinol. 156(9): 3137-3146. Saravanan, G; Kalaiselvi, .B. and Reddy, G.P.(2011). ''Hypolipidemic effect of zignidd (polyherbal formulation) in streptozotocin-induced diabetic rats''. INT. J. Drug Formulation and Research. 2(6): 151-160.20. Schacterle, G.R. and Pollack , R. L. (1973). ''A simplified method for the quantitatiue assay of small amounts of protein biological material''. J. Anal. Biochem. 51: 654-655. Shao, J.; Wang, Z.; Yang, T.; Ying, H.; Zhang, Y. and Liu, S. '' (2015). ''Bone regulates glucose metabolism as an endocrine organ through osteocalcin''. Internat. J. Endocrinol. 10: 1-9. Tomoda, M.; Shimizu, N.; Gonda, R.; Kanari, M.; Yamada, H. and Hikino, H. (1990). ''Anti-Complementary and hypoglycemic activities of the glycans from the seeds of malva verticillata''. J. Planta. Med. 56:168-170. Yeboah, J.O.; Owiredu, W.K.B.A.; Norgbe, G.K.; Lokpo, S.Y.; Gyamfi,J.; Allotey, E.A.; Aduko, R.A.; Noagbe, M. and Attah, F.A. (2017). '' The Prevalence of metabolic syndrome and its components among people with type 2 diabetes in the Ho Municipality, Ghana: A cross-sectional study''. Internat. J. Chronic Dis. 10: 1-8. Zoch, M.L.; Clemens, T.L. and Riddle, R.C. (2016). '' New insights into the biology of osteocalcin''. J. Bone and diabetes. 82: 42–49.
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Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Thikra Al- Allwsh

Liqa'a Abdulla

Publication Date November 24, 2019
Published in Issue Year 2019Volume: 7

Cite

APA Al- Allwsh, T., & Abdulla, L. (2019). Study the Effect of Isolated Osteocalcin from Human and Rats on Lipids Profile in Normal and Alloxan-Induced Diabetic Male Rats. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 7, 296-307.