Research Article
Year 2019,
Volume: 8 , 1 - 11, 10.12.2019
Abstract
References
- Nobili, S., Lippi, D., Witort, E., Donnini, M., Bausi, L., Mini, E., & Capaccioli, S. (2009). Natural compounds for cancer treatment and prevention. Pharmacological research, 59(6), 365-378. Uttara, B., Singh, A. V., Zamboni, P., & Mahajan, R. T. (2009). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current neuropharmacology, 7(1), 65-74. Carlson, S. E., Linder, H. P., & Donoghue, M. J. (2012). The historical biogeography of Scabiosa (Dipsacaceae): implications for Old World plant disjunctions. Journal of Biogeography, 39(6), 1086-1100. Bonet, M. À., Parada, M., Selga, A., & Valles, J. (1999). Studies on pharmaceutical ethnobotany in the regions of L’Alt Emporda and Les Guilleries (Catalonia, Iberian Peninsula). Journal of Ethnopharmacology, 68(1-3), 145-168. Bussmann, R. W., Glenn, A., Meyer, K., Kuhlman, A., & Townesmith, A. (2010). Herbal mixtures in traditional medicine in Northern Peru. Journal of Ethnobiology and Ethnomedicine, 6(1), 10. Bonet, M. À., & Valles, J. (2007). Ethnobotany of Montseny biosphere reserve (Catalonia, Iberian Peninsula): plants used in veterinary medicine. Journal of Ethnopharmacology, 110(1), 130-147. Hotta, M.; Ogata, K.; Nitta, A.; Hoshikawa, K.; Yanagi, M.; Yamazaki, K. Useful plants of the world, 1st ed.; Tokyo: Heibonsha LTD, 1989. Quezel P, Santa S (1963) Nouvelle flore de l’Algérie et des régions désertiques méridionales, vols 1–2. In: CNRS (Ed), Paris, pp 892 Bammi, J., & Douira, A. (2002). Medicinal plants in the forest of Achach (central plateau, Morocco). J. Acta Bot. Mal, 27, 131-145. Wannes, W. A., & Marzouk, B. (2016). Research progress of Tunisian medicinal plants used for acute diabetes. Journal of Acute Disease, 5(5), 357-363. Farnsworth, N. R. (1966). Biological and phytochemical screening of plants. Journal of pharmaceutical sciences, 55(3), 225-276. Le, K., Chiu, F., & Ng, K. (2007). Identification and quantification of antioxidants in Fructus lycii. Food Chemistry, 105(1), 353-363. Turkoglu, A., Duru, M. E., Mercan, N., Kivrak, I., & Gezer, K. (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry, 101(1), 267-273. Heimler, D., Vignolini, P., Dini, M. G., Vincieri, F. F., & Romani, A. (2006). Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food chemistry, 99(3), 464-469. Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. Oyaizu, M. (1986). Studies on products of browning reaction: antioxidative activity of products of browning reaction. Jpn. J. Nutr, 44(6), 307-315. Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26), 7970-7981. Decker, E. A., & Welch, B. (1990). Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and food Chemistry, 38(3), 674-677. Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. J., & McLaughlin, J. L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica, 45(05), 31-34. Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95. Medini, F., Fellah, H., Ksouri, R., & Abdelly, C. (2014). Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for science, 8(3), 216-224. Karthik, I. (2013). Evaluation of anti-inflammatory activity of Canthium parviflorum by in-vitro method. Al-Qudah, M. A., Otoom, N. K., Al-Jaber, H. I., Saleh, A. M., Abu Zarga, M. H., Afifi, F. U., & Abu Orabi, S. T. (2017). New flavonol glycoside from Scabiosa prolifera L. aerial parts with in vitro antioxidant and cytotoxic activities. Natural product research, 31(24), 2865-2874. Zheng, Q., Koike, K., Han, L. K., Okuda, H., & Nikaido, T. (2004). New Biologically Active Triterpenoid Saponins from Scabiosa t schiliensis. Journal of natural products, 67(4), 604-613. JI, M., LI, S. J., & MA, C. M. (2014). Chemical constituents of the inflorescence of Scabiosa comosa Fisch and their antioxide and α-glucosidase inhibitory activities. Journal of Inner Mongolia University (Natural Science Edition), 4, 398-403. Kuril'chenko, V. A., Zemtsova, G. N., & Bandyukova, V. Y. (1971). A chemical study of Scabiosa bipinnata. Chemistry of Natural Compounds, 7(4), 519-519. Christopoulou, C., Graikou, K., & Chinou, I. (2008). Chemosystematic value of chemical constituents from Scabiosa hymettia (Dipsacaceae). Chemistry & biodiversity, 5(2), 318-323. Lehbili, M., Magid, A. A., Hubert, J., Kabouche, A., Voutquenne-Nazabadioko, L., Renault, J. H.,Kabouche, Z. (2018). Two new bis-iridoids isolated from Scabiosa stellata and their antibacterial, antioxidant, anti-tyrosinase and cytotoxic activities. Fitoterapia, 125, 41-48. Lehbili, M., Magid, A. A., Kabouche, A., Voutquenne-Nazabadioko, L., Morjani, H., Harakat, D., & Kabouche, Z. (2018). Triterpenoid saponins from Scabiosa stellata collected in North-eastern Algeria. Phytochemistry, 150, 40-49. Rahmouni, N., Pinto, D. C., Santos, S. A., Beghidja, N., & Silva, A. M. (2018). Lipophilic composition of Scabiosa stellata L.: An underexploited plant from Batna (Algeria). Chemical Papers, 72(3), 753-762. Wang, J., Liu, K., Xu, D., Wang, Q., Bi, K., Song, Y., ... & Zhang, L. (2013). Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning. Plant growth regulation, 69(3), 305-310. Hlila, M. B., Mosbah, H., Mssada, K., Jannet, H. B., Aouni, M., & Selmi, B. (2015). Acetylcholinesterase inhibitory and antioxidant properties of roots extracts from the Tunisian Scabiosa arenaria Forssk. Industrial Crops and Products, 67, 62-69. Mouffouk, C., Mouffouk, S., Dekkiche, S., Hambaba, L., & Mouffouk, S. (2019). Antioxidant and Antibacterial Activities of the species Silene inflata Sm. PSM Biological Research, 4(2), 74-86. Mouffouk, S., Marcourt, L., Benkhaled, M., Boudiaf, K., Wolfender, J. L., & Haba, H. (2017). Two New Prenylated Isoflavonoids from Erinacea anthyllis with Antioxidant and Antibacterial Activities. Natural Product Communications, 12(7), 1934578X1701200716. Ayaz, M., Junaid, M., Ullah, F., Sadiq, A., Subhan, F., Khan, M. A., ... & Ahmad, S. (2016). Molecularly characterized solvent extracts and saponins from Polygonum hydropiper L. show high anti-angiogenic, anti-tumor, brine shrimp, and fibroblast NIH/3T3 cell line cytotoxicity. Frontiers in pharmacology, 7, 74. Mouffouk, C., Hambaba, L., Haba, H., Mouffouk, S., Bensouici, C., Hachemi, M., & Khadraoui, H. (2018). Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata. Oriental Pharmacy and Experimental Medicine, 18(4), 335-348. Mouffouk, C., Hambaba. L., Haba. H. , Mouffouk.,S , Bensouici.,C.(2018). Evaluation of Cytotoxic Effect, Anti-cholinesterase, Hemolytic and Antibacterial activities of the Species Scabiosa stellata L. Current Bioactive Compounds,10.2174/1573407214666180730102338 Wang, M., Guo, Q., Xu, X., Wang, X., Ye, X., Wu, S., ... & Wang, M. (2009). New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis. Antimicrobial agents and chemotherapy, 53(5), 1892-1897. Benli, M., Bingol, U., Geven, F., Guney, K., & Yigit, N. (2008). An Investigation on the antimicrobial activity of some endemic plant species from Turkey. African Journal of Biotechnology, 7(1).
Evaluation of cytotoxic Effect, anticholinesterase, antioxidant, antiarthritic and antibacterial activities of the Algerian species Scabiosa stellata L.
Abstract
The present study investigates the evaluation of in vitro pharmacological activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the plant Scabiosa stellata L. The cytotoxicity of extracts was tested by Brine shrimp lethality method; the acetylcholinesterase inhibitory activity was performed using Ellman's colorimetric method. The anti-arthritic activity was conducted by bovine serum albumin denaturation method, and the antioxidant activity was evaluated by six different methods including DPPH and ABTS radicals scavenging activities, reducing power, CUPRAC assay, ferrous ions and metal chelating assay. Furthermore, the antibacterial activity was estimated by agar disk diffusion assay against ten bacterial strains. The phytochemical screening of all the extracts revealed the presence of several types of secondary metabolites. The ethyl acetate extract recorded the highest content of polyphenols, flavonoids and tannins. All the crude extracts (PE, EtAOc and n-BuOH) had antioxidant activities in various assays and prevent the denaturation of bovin serum albumin in dose depending manner. A significant cytotoxic effect was observed for the n-butanolic extract with 57.2 ± 0.2% of mortality at 80 μg/mL, the ethyl acetate extract displayed a moderate anticholinesterase activity at 200 μg/mL. All the crude extracts showed antibacterial activity against most tested strains, with zones of inhibition ranging from 9 to 20 mm. The results indicate that the species S. stellata could be an important source of therapeutic agents against neurodegenerative inflammatory and infectious diseases.
References
- Nobili, S., Lippi, D., Witort, E., Donnini, M., Bausi, L., Mini, E., & Capaccioli, S. (2009). Natural compounds for cancer treatment and prevention. Pharmacological research, 59(6), 365-378. Uttara, B., Singh, A. V., Zamboni, P., & Mahajan, R. T. (2009). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current neuropharmacology, 7(1), 65-74. Carlson, S. E., Linder, H. P., & Donoghue, M. J. (2012). The historical biogeography of Scabiosa (Dipsacaceae): implications for Old World plant disjunctions. Journal of Biogeography, 39(6), 1086-1100. Bonet, M. À., Parada, M., Selga, A., & Valles, J. (1999). Studies on pharmaceutical ethnobotany in the regions of L’Alt Emporda and Les Guilleries (Catalonia, Iberian Peninsula). Journal of Ethnopharmacology, 68(1-3), 145-168. Bussmann, R. W., Glenn, A., Meyer, K., Kuhlman, A., & Townesmith, A. (2010). Herbal mixtures in traditional medicine in Northern Peru. Journal of Ethnobiology and Ethnomedicine, 6(1), 10. Bonet, M. À., & Valles, J. (2007). Ethnobotany of Montseny biosphere reserve (Catalonia, Iberian Peninsula): plants used in veterinary medicine. Journal of Ethnopharmacology, 110(1), 130-147. Hotta, M.; Ogata, K.; Nitta, A.; Hoshikawa, K.; Yanagi, M.; Yamazaki, K. Useful plants of the world, 1st ed.; Tokyo: Heibonsha LTD, 1989. Quezel P, Santa S (1963) Nouvelle flore de l’Algérie et des régions désertiques méridionales, vols 1–2. In: CNRS (Ed), Paris, pp 892 Bammi, J., & Douira, A. (2002). Medicinal plants in the forest of Achach (central plateau, Morocco). J. Acta Bot. Mal, 27, 131-145. Wannes, W. A., & Marzouk, B. (2016). Research progress of Tunisian medicinal plants used for acute diabetes. Journal of Acute Disease, 5(5), 357-363. Farnsworth, N. R. (1966). Biological and phytochemical screening of plants. Journal of pharmaceutical sciences, 55(3), 225-276. Le, K., Chiu, F., & Ng, K. (2007). Identification and quantification of antioxidants in Fructus lycii. Food Chemistry, 105(1), 353-363. Turkoglu, A., Duru, M. E., Mercan, N., Kivrak, I., & Gezer, K. (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry, 101(1), 267-273. Heimler, D., Vignolini, P., Dini, M. G., Vincieri, F. F., & Romani, A. (2006). Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food chemistry, 99(3), 464-469. Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. Oyaizu, M. (1986). Studies on products of browning reaction: antioxidative activity of products of browning reaction. Jpn. J. Nutr, 44(6), 307-315. Apak, R., Güçlü, K., Özyürek, M., & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26), 7970-7981. Decker, E. A., & Welch, B. (1990). Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and food Chemistry, 38(3), 674-677. Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. J., & McLaughlin, J. L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica, 45(05), 31-34. Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95. Medini, F., Fellah, H., Ksouri, R., & Abdelly, C. (2014). Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for science, 8(3), 216-224. Karthik, I. (2013). Evaluation of anti-inflammatory activity of Canthium parviflorum by in-vitro method. Al-Qudah, M. A., Otoom, N. K., Al-Jaber, H. I., Saleh, A. M., Abu Zarga, M. H., Afifi, F. U., & Abu Orabi, S. T. (2017). New flavonol glycoside from Scabiosa prolifera L. aerial parts with in vitro antioxidant and cytotoxic activities. Natural product research, 31(24), 2865-2874. Zheng, Q., Koike, K., Han, L. K., Okuda, H., & Nikaido, T. (2004). New Biologically Active Triterpenoid Saponins from Scabiosa t schiliensis. Journal of natural products, 67(4), 604-613. JI, M., LI, S. J., & MA, C. M. (2014). Chemical constituents of the inflorescence of Scabiosa comosa Fisch and their antioxide and α-glucosidase inhibitory activities. Journal of Inner Mongolia University (Natural Science Edition), 4, 398-403. Kuril'chenko, V. A., Zemtsova, G. N., & Bandyukova, V. Y. (1971). A chemical study of Scabiosa bipinnata. Chemistry of Natural Compounds, 7(4), 519-519. Christopoulou, C., Graikou, K., & Chinou, I. (2008). Chemosystematic value of chemical constituents from Scabiosa hymettia (Dipsacaceae). Chemistry & biodiversity, 5(2), 318-323. Lehbili, M., Magid, A. A., Hubert, J., Kabouche, A., Voutquenne-Nazabadioko, L., Renault, J. H.,Kabouche, Z. (2018). Two new bis-iridoids isolated from Scabiosa stellata and their antibacterial, antioxidant, anti-tyrosinase and cytotoxic activities. Fitoterapia, 125, 41-48. Lehbili, M., Magid, A. A., Kabouche, A., Voutquenne-Nazabadioko, L., Morjani, H., Harakat, D., & Kabouche, Z. (2018). Triterpenoid saponins from Scabiosa stellata collected in North-eastern Algeria. Phytochemistry, 150, 40-49. Rahmouni, N., Pinto, D. C., Santos, S. A., Beghidja, N., & Silva, A. M. (2018). Lipophilic composition of Scabiosa stellata L.: An underexploited plant from Batna (Algeria). Chemical Papers, 72(3), 753-762. Wang, J., Liu, K., Xu, D., Wang, Q., Bi, K., Song, Y., ... & Zhang, L. (2013). Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning. Plant growth regulation, 69(3), 305-310. Hlila, M. B., Mosbah, H., Mssada, K., Jannet, H. B., Aouni, M., & Selmi, B. (2015). Acetylcholinesterase inhibitory and antioxidant properties of roots extracts from the Tunisian Scabiosa arenaria Forssk. Industrial Crops and Products, 67, 62-69. Mouffouk, C., Mouffouk, S., Dekkiche, S., Hambaba, L., & Mouffouk, S. (2019). Antioxidant and Antibacterial Activities of the species Silene inflata Sm. PSM Biological Research, 4(2), 74-86. Mouffouk, S., Marcourt, L., Benkhaled, M., Boudiaf, K., Wolfender, J. L., & Haba, H. (2017). Two New Prenylated Isoflavonoids from Erinacea anthyllis with Antioxidant and Antibacterial Activities. Natural Product Communications, 12(7), 1934578X1701200716. Ayaz, M., Junaid, M., Ullah, F., Sadiq, A., Subhan, F., Khan, M. A., ... & Ahmad, S. (2016). Molecularly characterized solvent extracts and saponins from Polygonum hydropiper L. show high anti-angiogenic, anti-tumor, brine shrimp, and fibroblast NIH/3T3 cell line cytotoxicity. Frontiers in pharmacology, 7, 74. Mouffouk, C., Hambaba, L., Haba, H., Mouffouk, S., Bensouici, C., Hachemi, M., & Khadraoui, H. (2018). Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata. Oriental Pharmacy and Experimental Medicine, 18(4), 335-348. Mouffouk, C., Hambaba. L., Haba. H. , Mouffouk.,S , Bensouici.,C.(2018). Evaluation of Cytotoxic Effect, Anti-cholinesterase, Hemolytic and Antibacterial activities of the Species Scabiosa stellata L. Current Bioactive Compounds,10.2174/1573407214666180730102338 Wang, M., Guo, Q., Xu, X., Wang, X., Ye, X., Wu, S., ... & Wang, M. (2009). New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis. Antimicrobial agents and chemotherapy, 53(5), 1892-1897. Benli, M., Bingol, U., Geven, F., Guney, K., & Yigit, N. (2008). An Investigation on the antimicrobial activity of some endemic plant species from Turkey. African Journal of Biotechnology, 7(1).
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| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 10, 2019 |
| Published in Issue | Year 2019Volume: 8 |
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