Helicobacter pylori is the pathogen only known that inhabits the gastric mucosa of almost half of the world’s population, and the bacterium is associated with higher incidence of peptic ulcer worldwide. The present study was aimed to seek for diagnosis of H. pylori as etiological agents of peptic ulcer in Iraqi patients on second line therapy and suffering from severe ulcer reinfection after a period of time. Sixty-five endoscopic gastric biopsy specimens were obtained from patients of both genders and in age around 45-60 years. For primary isolation, 26 (40%) out of all corpus and antrum screened biopsies were positive on supplemented Columbia agar. Culture isolates showed heteroresistance pattern to antibiotics used in triple therapy regimen for eradication of H. pylori infections, in that high percentage of resistance to tetracycline and metronidazole (100%) was recorded, while most of isolates were sensitive and in various degrees (27%, 12.5%, and 25%) to amoxicillin, clarithromycin, and levofloxacin respectively, on the other hand one isolate exhibited absolute resistance to all of the tested antibiotics. The molecular detection of 16S rRNA (109bp) and ureA (411bp) genes specific for H. pylori were not detected by PCR amplification. Two isolates which showed significant similarities to H. pylori throughout the morphological and cultural examination, were selected and subjected to molecular analysis via 16S DNA sequencing. GenBank BLAST analysis was showed that the isolates were non-H. pylori isolates, rather, one was identified as Achromobacter xylosoxidans and the other Klebsiella oxytoca, with 98% and 97% identical gene sequences respectively.
Helicobacter pylori Colombia agar medium Standard triple therapy Proteobacteria Klebsiella oxytoca Achromobacter xylosoxidans
Primary Language | English |
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Subjects | Engineering |
Journal Section | Articles |
Authors | |
Publication Date | June 29, 2020 |
Published in Issue | Year 2020Volume: 9 |