Abstract
Allergy is an important health problem affecting public health. Allergic diseases occur when the immune system reacts to non-harmful substances with the effect of genetic predisposition and environmental factors. According to the data of the World Allergy Organization (WAO), the prevalence of allergies in different countries varies between 10-40%. Pollen, mold, animal hair, house dust mite, medicines, and foods are the most common allergen agents. Common mushrooms in nature have the potential to produce allergenic proteins. Penicillium, Aspergillus, Rhizopus, and Mucor species, which are allergic fungi, are widely found in nature. In our country, 614 patients with respiratory allergy have been reported to develop allergic reactions against Aspergillus fumigatus, Trichophyton rubrum, Mucor, Penicillium notatum, Aspergillus niger, and Alternaria tenius. In recent years, the cases of allergies caused by molds have increased significantly and studies to determine the causing allergens have accelerated. Mucor mucedo (brown bread mold) was used in our study. Mucor mucedo produced in our laboratory was collected and allergen fungus protein was extracted by 2 different extraction methods. By preparing protein samples from prepared mushroom extracts, the total concentration of potential allergen proteins was determined by the BCA method. According to the data obtained, it was determined that the protein concentration of the mushroom samples dried by that were subjected to dialysis was higher than ethanol. As a result, Mucor mucedo was found to have a high protein concentration and revealed basic data for further analysis.
Keywords
References
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Abstract
References
- Actor, J. K. (2011). Elsevier's Integrated Review Immunology and Microbiology (pp. 139-146) E-Book: with Student Consult Online Access. Elsevier Health Sciences. http://www.sciencedirect.com/science /article/pii/B9780323074476000156
- Çeter, T. Y., & Pinar, N. M. (2008). The concentration of atmospheric pollen and spores of Kastamonu province (Central Region) and effects of meteorological factors on this concentration (January 2006- December 2007) (Doctoral dissertation, Ankara University Graduate School of Natural and Applied Sciences Department of Biology.
- Esch, R. E., & Codina, R. (2017). Fungal raw materials used to produce allergen extracts. Annals of allergy, asthma & immunology : Official publication of the American College of Allergy, Asthma, & Immunology, 118(4), 399–405. https://doi.org/10.1016/j.anai.2016.05.012
- Güneser, S., Atici, A., Köksal, F., & Yaman, A. (1994). Mold allergy in Adana, Turkey. Allergologia et immunopathologia, 22(2), 52–54.
- Kendrick B. (2000). The Fifth Kingdom (pp. 227-247). Hackett Publishing
- Money, N. P. (2016). Fungal cell biology and development. In The fungi (pp. 37-66). Academic Press.
- Pawankar, R., Canonica, G. W., Holgate, S. T., Lockey, R. F., & Blaiss, M. S. (2011). WAO white book on allergy (pp. 102-110). Milwaukee, WI: World Allergy Organization, 3, 156-157.
- Simon-Nobbe, B., Denk, U., Pöll, V., Rid, R., & Breitenbach, M. (2008). The spectrum of fungal allergy. International archives of allergy and immunology, 145(1), 58–86. https://doi.org/10.1159/000107578
- Şimşekli, Y. (1994). The fungus exist in the air outside the hauses in the different regions of Bursa. (Master's thesis, Uludağ University.
- Tao, A., & Raz, E. (Eds.). (2015). Allergy bioinformatics (8nd ed., pp.1-14). Springer.
- Tatlıdil, S., Bıçakçı, A., Akkaya, A, & Malyer, H. (2001). Allergen cladosporium sp. and alternaria sp. spores in the atmosphere of Burdur. Süleyman Demirel University Medical Faculty Journal:8(4), 1-3.
- Walker J M. 2002. The Protein Protocols Handbook (pp. 11-14). Second edition. Humana Press.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 31, 2020 |
| Published in Issue | Year 2020Volume: 10 |
