Conference Paper
Extraction and Purification of the Potential Allergen Proteins from Candida Albicans
Abstract
A commercial strain of Candida albicans10231 was grown and isolated using Sabouraud's Dextrose Agar Media. The samples were then subjected to lyophilization before protein extraction. TCA/acetone/methanol/phenol method has been used for protein extraction. For estimation of the percentage of extracted protein, BCA Protein Macro Assay Kit was applied. The samples were then transferred to the spectrophotometer for reading at wave length of 562 nm. The results show that the profile of the quantity of protein concentrations obtained upon supplementation of the following chemicals: PBS, ammonium bicarbonate, and 50mM of SDS buffer, were 0.133mg/mL, 0.080mg/mL, and 0.068mg/mL respectively.
References
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- Kustrzeba-Wójcicka, I., Siwak, E., Terlecki, G., Wolańczyk-Mędrala, A., & Mędrala, W. (2014). Alternaria alternata and its allergens: a comprehensive review. Clinical Reviews in Allergy & Immunology, 47(3), 354–365. https://doi.org/10.1007/s12016-014-8447-6
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- Schillinger, J. E., Vu, T., & Bellin, P. (1999). Airborne fungi and bacteria background levels in office buildings. Journal of Environmental Health, 62(2), p. 9–14.
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Abstract
References
- Aydoğdu, H, & Asan A. (2008). Airborne fungi in child day care centers in Edirne City, Turkey. Environ Monit Assess; 147, 423-44. http://dx.doi.org/10.1007/s10661-007-0130-4
- Bahnan, W., Koussa, J., Younes, S., Abi Rizk, M., Khalil, B., El Sitt, S., et al (2012). Deletion of the Candida albicans PIR32 results in increased virulence, stress response, and upregulation of cell wall chitin deposition. Mycopathologia, 174, 107–19. https://doi.org/10.1007/s11046-012-9533-z PMID: 22391823
- Bezerra, G. F. D. B., Gomes, S. M., Silva, M. A. C. N. D., Santos, R. M. D., Muniz Filho, W. E., Viana, G. M. D. C., & Nascimento, M. D. D. S. B. (2014). Diversity and dynamics of airborne fungi in São Luis, State of Maranhão, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 47(1), 69-73.
- Carlile, M. J., Watkinson, S. C., & Gooday, G. W. (2001). The fungi. Gulf Professional Publishing.
- Chakrabarti, H. S., Das, S., & Gupta-Bhattacharya, S. (2012). Outdoor airborne fungal spora load in a suburb of Kolkata, India: its variation, meteorological determinants and health impact. International Journal of Environmental Health Research, 22(1), 37-50.
- Fang, Z., Ouyang, Z., Hu, L., & Wang, X. Z. H, LX. (2005). Culturable airborne fungi in outdoor environments in Beijing. China. Science and Total Environment, 350, 47-58.
- Grinn-Gofroń, A. (2011). Airborne Aspergillus and Penicillium in the atmosphere of Szczecin, (Poland) (2004-2009). Aerobiologia, 27(1), 67–76. https://doi.org/10.1007/s10453-010-9177-8
- Günenç, M.Z. (2020, 06 May). What is a vaginal yeast infection?. https://www.florence.com.tr/vajinal-mantar
- Kapteyn, J. C., Hoyer, L. L., Hecht, J. E., Müller, W. H., Andel, A., Verkleij, A. J., ... & Klis, F. M. (2000). The cell wall architecture of Candida albicans wild‐type cells and cell wall‐defective mutants. Molecular Microbiology, 35(3), 601-611.
- Kustrzeba-Wójcicka, I., Siwak, E., Terlecki, G., Wolańczyk-Mędrala, A., & Mędrala, W. (2014). Alternaria alternata and its allergens: a comprehensive review. Clinical Reviews in Allergy & Immunology, 47(3), 354–365. https://doi.org/10.1007/s12016-014-8447-6
- Martínez, A. I., Castillo, L., Garcera, A., Elorza, M. V., Valentín, E., & Sentandreu, R. (2004). Role of Pir1 in the construction of the Candida albicans cell wall. Microbiology, 150(10), 3151-3161.
- Schillinger, J. E., Vu, T., & Bellin, P. (1999). Airborne fungi and bacteria background levels in office buildings. Journal of Environmental Health, 62(2), p. 9–14.
- Töre, O., (1996). Basic microbiology and parasitology, chapter 14.general mycology (Editor: Kılıçturgay, K.) Güneş & Nobel Medical Bookstores, Bursa-İstanbul.
There are 13 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 31, 2020 |
| Published in Issue | Year 2020 Volume: 10 |
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