Conference Paper
Year 2021,
Volume: 12 , 62 - 66, 31.12.2021
Abstract
Pollen forms large amount of aeroallergens in the atmosphere that spread through winds and insects. Allergenic pollen grains are water-soluble glycoprotein or protein bioparticles and its weight is approximately 5-80 kDa. There are many factors that cause allergies and considering the recently changing climatic conditions and their ability to spread over a wide geographical areas, pollens are among the major factors causing allergies. In this study, it was aimed to detect potential allergen pollen proteins of Tilia cordata which is a potent allergen. The pollen samples belonging to T. cordata were dried and then they were separated with sieve. Acetone washing and dialysis processes were applied to purificate pollen. Afterwards, the amount of protein in the pollens of T. cordata was determined by the bicinchoninic acid (BCA) method. In order to detect allergen proteins, gel runing was performed by SDS-PAGE. Silver staining method was applied to make visible the bands of T. cordata pollen proteins obtained from the gel. Five different allergenic pollen proteins, weights 10, 23, 40, 50 and 80 kDa, were detected as a result of SDS-PAGE. This is the second study in the literature related with the identification of T. cordata allergenic proteins and we showed two different protein bands compared with the former study result. Besides, our study is original in this perspective. We think that this study contributes to the literature on allergenic proteins of T. cordata and should be supported by future studies.
References
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- Pleasants, J. M., Hellmich, R. L., Dively, G. P., Sears, M. K., Stanley-Horn, D. E., Mattila, H. R., ... & Jones, G. D. (2001). Corn pollen deposition on milkweeds in and near cornfields. Proceedings of the National Academy of Sciences, 98(21), 11919-11924.
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- Wodehouse R.P. (1935). Pollen Grains. New York: McGraw-Hill.
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Year 2021,
Volume: 12 , 62 - 66, 31.12.2021
Abstract
References
- Aytug, B., & Peremeci, E. (1987). Polen, saman nezlesi ve polen ekstreleri. IÜ Tıp Fakültesi Mecmuası, 50, 163-170.
- Chinen J, Fleisher TA, Shearer WT. (2009). The immune system: an overview. In: Adkinson Jr NF, Bochner BS, Busse WW, Holgate ST, Lemanske RF, Simons EF, editors. Middleton’s Allergy. Principles and Practice. 7th ed. Mosby: Elsevier.
- Pigott, C. D. (1991). Tilia cordata miller. Journal of Ecology, 79(4), 1147-1207.
- D’amato, G., Cecchi, L., Bonini, S., Nunes, C., Annesi‐Maesano, I., Behrendt, H., ... & Van Cauwenberge, P. (2007). Allergenic pollen and pollen allergy in Europe. Allergy, 62(9), 976-990.
- Furness, C. A., & Rudall, P. J. (2001). Pollen and anther characters in monocot systematics. Grana, 40(1-2), 17-25.
- Esch, R. E., Hartsell, C. J., Crenshaw, R., & Jacobson, R. S. (2001). Common allergenic pollens, fungi, animals, and arthropods. Clinical reviews in allergy & immunology, 21(2), 261-292.
- Gamble, J. L., Reid, C. E., Post, E., & Sacks, J. (2008). Review of the impacts of climate variability and change on aeroallergens and their associated effects. Washington, DC: US Environmental Protection Agency.
- Grote, M., Vrtala, S., Niederberger, V., Wiermann, R., Valenta, R., & Reichelt, R. (2001). Release of allergen-bearing cytoplasm from hydrated pollen: a mechanism common to a variety of grass (Poaceae) species revealed by electron microscopy. Journal of Allergy and Clinical Immunology, 108(1), 109-115.
- Jae-Won O., (2018). Pollen Allergy in a Changing World (pp. 165-172). Springer.
- Te Piao King, H. D., Lowenstem, H., & Marsh, D. G. (1995). Allergen nomenclature. Allergy, 50, 765-774.
- Mur, P., Feo Brito, F., Lombardero, M., Barber, D., Galindo, P. A., Gomez, E., & Borja, J. (2001). Allergy to linden pollen(Tilia cordata). Allergy, 56(5), 457-458.
- Pehlivan, S. (1984). Aeropalinolojik çalışmaların tıptaki önemi”. Türk Hijyen ve Deneysel Biyoloji Dergisi, 41(3), 315-323.
- Pleasants, J. M., Hellmich, R. L., Dively, G. P., Sears, M. K., Stanley-Horn, D. E., Mattila, H. R., ... & Jones, G. D. (2001). Corn pollen deposition on milkweeds in and near cornfields. Proceedings of the National Academy of Sciences, 98(21), 11919-11924.
- Radauer, C., Bublin, M., Wagner, S., Mari, A., & Breiteneder, H. (2008). Allergens are distributed into few protein families and possess a restricted number of biochemical functions. Journal of allergy and clinical immunology, 121(4), 847-852.
- Stewart GA. (2000). The molecular biology of allergens. In: Busse WW, Holgate ST, editors. Asthma and Rinitis. (pp. 1107–42). Boston: Blackwell Scientific;.
- Traidl-Hoffmann, C., Jakob, T., & Behrendt, H. (2009). Determinants of allergenicity. Journal of Allergy and Clinical Immunology, 123(3), 558-566.
- Krüger, N. J., & Walker, J. M. (2002). The Bradford method for protein quantification. The Protein Protocols Handbook (2nd Edition). Humana Press Inc., New Jersey, 15-22.
- Wodehouse R.P. (1935). Pollen Grains. New York: McGraw-Hill.
- Pincus, M. R., Abraham, N. Z., Carty, R. P., & McPherson, R. A. (2011). Clinical enzymology. Henry’s Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Saunders, Philadelphia, 273-295.
There are 19 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Early Pub Date | September 5, 2021 |
| Publication Date | December 31, 2021 |
| Published in Issue | Year 2021Volume: 12 |
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