Investigation of Antimicrobial, DNA Protective and Cytotoxic Activity of Red Cabbage (Brassica Oleracea L. Var. Capitata F. Rubra) Plant
Abstract
The need to search for natural products that offer a safe alternative to drugs and industrial products that often cause side effects and harm human health is growing over the years. In this study, the water and methanol extracts of the anthocyanin-rich red cabbage (Brassica oleracea L. var. capitata f. rubra) which is available on markets throughout the year, were examined for their antibacterial activity on Escherichia coli, Staphylococcus aereus and Stenotrophomonas maltophilia, and for their antifungal activity on Yarrowia lipolytica, Candida albicans and Candida maltophilia. The DNA protective activity of extracts on the pBR322 plasmid was evaluated by exposing pBR322 to UV and adding hydrogen peroxide. Furthermore, the MTT assay was performed on the H1299 lung cancer cell line and the HUVEC cell line as a negative control to evaluate the cytotoxicity of the extracts. The red cabbage extracts were found to have no antibacterial or antifungal activity on the tested microorganisms. The results of DNA protective activity have shown that at a concentration of 50 mg/mL there is a DNA protective activity of the extracts. No effect of the red cabbage extracts on the H1299 cell line as shown by MTT assay results. On the other hand, the decrease in the viability of HUVEC cells started at a concentration of 25 μg/mL and above, reaching 70% at 100 μg/ mL for the water extract and 74 % at 100 μg/ mL for the methanol extract. Further studies to investigate the active components of the DNA protective activity in the red cabbage extracts are required. Further studies are also needed to explore the possibility of incorporating these components in cosmetic products such as sunscreens without causing skin damage.
References
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- Bahuguna, A., Khan, I., Bajpai, V.K., Kang, S.C. (2017). MTT assay to evaluate the cytotoxic potential of a drug, Bangladesh Journal of Pharmacology. 12(2), 115-118.
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- Hafidh, R. R., Abdulamir, A. S., Vern, L. S., Bakar, F. A., Abas, F., Jahanshiri, F., Sekawi, Z. (2011). Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product, The Open Microbiology Journal. 5(1), 96-106.
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- Kilic, B. (2020). ‘’Yeni sentez platin (II) sakkarinat kompleksinin (trans [PT (sac) 2 (PPH2CY) 2]) panc-1 pankreas kanseri hücreleri üzerindeki sitotoksik/apoptotik etkilerinin araştırılması’’ Yüksek Lisans Tezi, Sağlık Bilimleri Enstitüsü, İstinye Üniversitesi, İstanbul.
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- Tajalli, F., Saeedi, M., Malekabadi, A. V. (2020). Anticancer and antioxidant effects of red cabbage on three cancerous cell lines and comparison with a normal cell line (HFF-3). Journal of Genes and Cells, 6(1), 12-20.
Abstract
References
- Ayshwarya, M., & Sudha Rameshwari, K. (2015). Antimicrobial activity of the plant extracts of Brassica oleracea var. Capitata rubra. Journal of International Academic Research for Multidisciplinary, 3, 149-156.
- Bahuguna, A., Khan, I., Bajpai, V.K., Kang, S.C. (2017). MTT assay to evaluate the cytotoxic potential of a drug, Bangladesh Journal of Pharmacology. 12(2), 115-118.
- Devi, J. R., & Thangam, E. B. (2012). Mechanisms of anticancer activity of sulforaphane from Brassica oleracea in HEp-2 human epithelial carcinoma cell line. Asian Pacific Journal of Cancer Prevention, 13(5), 2095-2100.
- Draghici, G. A., Alexandra, L. M., Aurica–Breica, B., Nica, D., Alda, S., Liana, A., ... & Despina-Maria, B. (2013). Red cabbage, millennium’s functional food. Journal of Horticulture, Forestry, and Biotechnology, 17(4), 52-55.
- ‘’EUCAST disk diffusion method for antimicrobial susceptibility testing’’. EUCAST.https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_test_documents/2021_manuals/Reading_guide_v_8.0_EUCAST_Disk_Test_2021.pdf, 04.02.2021.
- Farag, M.A., Abdel Motaal, A. (2010). Sulforaphane composition, cytotoxic and antioxidant activity of crucifer vegetables, Journal of Advanced Research. 1(1), 65-70.
- Fernandes, D. L., Hedge, K., Shabaraya, A.R. (2019). SCIENTIFIC APPROACHES ON RED CABBAGE: A REVIEW. International Journal of Pharma And Chemical Research, 5(3), 123-130.
- Hafidh, R. R., Abdulamir, A. S., Vern, L. S., Bakar, F. A., Abas, F., Jahanshiri, F., Sekawi, Z. (2011). Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product, The Open Microbiology Journal. 5(1), 96-106.
- Hafidh, R. R., Abdulamir, A. S., Bakar, F. A., Jalilian, F. A., Jahanshiri, F., Abas, F., & Sekawi, Z. (2013). Novel anticancer activity and anticancer mechanisms of Brassica oleracea L. var. capitata f. rubra. European Journal of Integrative Medicine, 5(5), 450-464.
- Kada, S., Bouriche, H., Senator, A., Demirtaş, I., Özen, T., Çeken Toptanci, B., Kızıl, G., Kızıl M. (2017) Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation, Pharmaceutical biology, 55(1), 330-337.
- Kilic, B. (2020). ‘’Yeni sentez platin (II) sakkarinat kompleksinin (trans [PT (sac) 2 (PPH2CY) 2]) panc-1 pankreas kanseri hücreleri üzerindeki sitotoksik/apoptotik etkilerinin araştırılması’’ Yüksek Lisans Tezi, Sağlık Bilimleri Enstitüsü, İstinye Üniversitesi, İstanbul.
- Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews, 4(8), 118.
- Santhosh, T., Vaithiyanathan, T. (2018). Antibacterial activity of Brassica oleracea L. var. Capitata F. Rubra Natural Dye, European Journal Of Biomedical And Pharmaceutical Sciences. 5(9), 332-335.
- Tajalli, F., Saeedi, M., Malekabadi, A. V. (2020). Anticancer and antioxidant effects of red cabbage on three cancerous cell lines and comparison with a normal cell line (HFF-3). Journal of Genes and Cells, 6(1), 12-20.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Early Pub Date | September 5, 2021 |
| Publication Date | December 31, 2021 |
| Published in Issue | Year 2021Volume: 12 |
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