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Investigation of the Role of Vitamin C In Enhancing the Activity of Antibacterial Agents and Biofilm Formation

Year 2021, Volume 16, Issue , 195 - 203, 31.12.2021


Resistance to antibiotics is rapidly spreading across the globe, posing a new health-care challenge for all countries. Bacterial biofilms are three-dimensional formations made up of cells encased in a matrix of polymeric, making cells resistant to the drugs and the immune system. As a result, new tactics for inhibiting the production of the EPS matrix may lead to more efficient use of already available antibiotics. The mechanism of vitamins C effect on boosting the effectiveness of several anti-bacterial drugs was investigated in this study, the target isolates were obtained from University of Mosul/ Biology department/ bacterial culture collections and evaluated qualitatively and quantitatively. The isolates involved: Staphylococcus aureus, Escherichia coli ,Klebsiella sp., Serratia marcescens and Pseudomonas aeroginosa. Antibiotic sensitivity tests and biofilm producing assay results revealed that the majority of the isolates were resistant to a range of antibiotics and had a large capacity for biofilm formation when grown on a cover glass surface. Vitamin C is an antioxidant, a scavenger of active metabolites. The Minimum Inhibitory Concentration (MIC) of Vitamin C against selected isolates had been determined, and all further experiments used concentrations below the MIC. Our results showed that Vit.C pre-treatment enhance the bactericidal effect of antibiotic and increases bacterial susceptibility to antibiotics. Using light microscopy, experiments of sub inhibitory doses of Vitamin C revealed good suppression of selected isolates biofilm development on the cover glass surface. Vitamin C can be utilized as an antibiotic adjuvant in combination with antibiotic and has effective biofilm inhibition, caused by multidrug-resistant bacteria, according to evidence.


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Primary Language English
Subjects Engineering
Journal Section Articles

Khansa MOHAMMED YOUNIS This is me
University of Mosul

Ghaydaa M. A. ALI This is me
University of Mosul

Ashwaq Hazem NAJEM This is me
University of Mosul

Publication Date December 31, 2021
Published in Issue Year 2021, Volume 16, Issue


APA Mohammed Younıs, K. , M. A. Alı, G. & Najem, A. H. (2021). Investigation of the Role of Vitamin C In Enhancing the Activity of Antibacterial Agents and Biofilm Formation . The Eurasia Proceedings of Science Technology Engineering and Mathematics , 16 , 195-203 . DOI: 10.55549/epstem.1068610