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Measurements the Level of Lipid Peroxidation and Some Antioxidants in Blood Serum of Thalassemia’s Patients

Year 2021, Volume 16, Issue , 225 - 230, 31.12.2021
https://doi.org/10.55549/epstem.1068616

Abstract

Repeated blood transfusion in beta thalassemia patients may lead to peroxidative tissue injury by secondary iron overload. In the present study, (43) patients with beta thalassemia. We have evaluated hemoglobin (Hb), packed cell volume (PCV), red blood cells (RBC), white blood cells (WBC), iron (Fe), ferriten, uric acid, glutathion (GSH), malondialdehyde (MDA), Vitamins C and E and electrolytes as sodium (Na), potassium (K), and chloride (Cl). The findings were compared with (25) age matched healthy individuals were included in this study as a control group. A significant increase in the levels of WBC, Fe, ferritin, MDA and Vit C (P < 0.001), whereas significant decrease in the levels of Hb, PCV, RBC, GSH and Vit E (P < 0.001) was observed. Uric acid, Na and K were significant increase (P < 0.05) in the patients when compared with controls, while there was a non-significant increase in mean value of Cl. These results were suggesting that oxidative stress and reduced antioxidant defense mechanism play an important role in pathogenesis of beta thalassemia major. We can conclude that defective membrane transport is responsible for observed changes of lipid peroxidation and some antioxidants. These results may help to understand the altered electrolyte homeostasis in thalassemia but there is still need of many future studies to clarify their mechanism of generation and pathological significance.

References

  • Al- Janabi, G. , Al-Fahham, A. & Mohammed, R. (2021). Pathophysiology of sickle cell anemia: review article. Indian Journal of Forensic Medicine and Toxicology, 15(2), 2037-2043.
  • Piga, A., Perrotta, S., Gamberini, M. R., Voskaridou, E., Melpignano, A., Filosa, A., ... & Attie, K. M. (2019). Luspatercept improves hemoglobin levels and blood transfusion requirements in a study of patients with β-thalassemia. Blood, The Journal of the American Society of Hematology, 133(12), 1279-1289.
  • Annino, J. S. & Giese, R. W. (1976). Clinical the chemistry: principles and procedures (4th ed.). Little Brown and Co.
  • Abo-Shanab, A. M., Kholoussi, N., Habib, D., Helwa, I. A., Hussin, G., Omar, N., ... & Fahmy, A. (2020). Use of echocardiography and glutathione S-transferase to detect heart complications in β-thalassemic patients. Egyptian Pharmaceutical Journal, 19(3), 244.
  • Kingchaiyaphum, B., Sanchaisuriya, K., Fucharoen, G., Chaibunruang, A., Hess, S. Y., Hinnouho, G. M., ... & Fucharoen, S. (2020). Hemoglobins F, A2, and E levels in Laotian children aged 6‐23 months with Hb E disorders: Effect of age, sex, and thalassemia types. International Journal of Laboratory Hematology, 42(3), 277-283.
  • Rund, D. (2016). Thalassemia 2016: modern medicine battles an ancient disease. American Journal of Hematology, 91(1), 15-21.
  • Anselmo, F. C., Ferreira, N. S., Mota, A. J. D., Gonçalves, M. D. S., Albuquerque, S. R. L., Fraiji, N. A., ... & Moura Neto, J. P. D. (2020). Deletional alpha-thalassemia alleles in amazon blood donors. Advances in Hematology, 2020, 1-6. https://doi.org/10.1155/2020/4170259.
  • Hinton, P. R. (2004). Statistical explained (2nd Edition). Rontledge.
  • Motta, I., Bou-Fakhredin, R., Taher, A. T., & Cappellini, M. D. (2020). Beta thalassemia: new therapeutic options beyond transfusion and iron chelation. Drugs, 80(11), 1053-1063.
  • Kadiiska, M. B., Peddada, S., Herbert, R. A., Basu, S., Hensley, K., Jones, D. P., ... & Mason, R. P. (2015). Biomarkers of oxidative stress study VI. Endogenous plasma antioxidants fail as useful biomarkers of endotoxin-induced oxidative stress. Free Radical Biology and Medicine, 81, 100-106.
  • Srinoun, K., Sathirapongsasuti, N., Paiboonsukwong, K., Sretrirutchai, S., Wongchanchailert, M., & Fucharoen, S. (2019). miR-144 regulates oxidative stress tolerance of thalassemic erythroid cell via targeting NRF2. Annals of hematology, 98(9), 2045-2052.
  • Sirirat, K., Sriwantana, T., Kaewchuchuen, J., Paiboonsukwong, K., Fucharoen, S., Ritthidej, G., ... & Sibmooh, N. (2019). Pharmacokinetics and pharmacodynamics of single dose of inhaled nebulized sodium nitrite in healthy and hemoglobin E/β-thalassemia subjects. Nitric Oxide, 93, 6-14.
  • Choudhary, M., Vyas, R. K., & Lahoti, A. (2017). Correlation of oxidative stress with serum antioxidant enzymes level in thalassemia in a tertiary level hospital of western Rajasthan. International Journal of Biotechnology and Biochemistry, 13(2), 155-65.
  • Khoshfetrat, M. R., Mortazavi, S., Neyestani, T., Mahmoodi, M. R., Zerafati-Shoae, N., & Mohammadi-Nasrabadi, F. (2014). Iron and vitamin C Co-Supplementation increased serum vitamin C without adverse effect on zinc level in iron deficient female youth. International Journal of Preventive Medicine, 5(8), 1037.
  • Hashim, N. A., Abdullah, Y. J., & Ibadi, H. A. (2020). Evolution of some biochemical and hematological parameters of thalassemia patients in Maysan Governorate, Iraq. Annals of Tropical Medicine and Public Health, 23, 231-238.
  • Psatha, N., Reik, A., Phelps, S., Zhou, Y., Dalas, D., Yannaki, E., ... & Papayannopoulou, T. (2018). Disruption of the BCL11A erythroid enhancer reactivates fetal hemoglobin in erythroid cells of patients with β-thalassemia major. Molecular Therapy-Methods & Clinical Development, 10, 313-326.
  • Sandro, A., Naidu, M., Philipp, S., Timm, S., Michaela, A., Axel, W., Katarzyna, O., Lothar, H., Manuela, S., Camilla, L., Cornelia, K., Josef, M., Dirk, J., Frauke, N., Birgit, R., Martin, H., Christian, B., Katja, M., Marcus, C., Tomas, G., Manfred, K., Martina, U. & Georg, W. (2020). Glutathione peroxidase 4 and vitamin E control reticulocyte maturation, stress erythropoiesis and iron homeostasis. Haematologica,105(4), 937–950.
  • Sedlak, J. & Lindsay, R. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical biochemistry, 25, 192-205. https://doi.org/10.1016/0003-2697(68)90092-4.
  • Sobhani, S., Rahmani, F., Rahmani, M., Askari, M., & Kompani, F. (2019). Serum ferritin levels and irregular use of iron chelators predict liver iron load in patients with major beta thalassemia: a cross-sectional study. Croatian Medical Journal, 60(5), 405-413. https://doi.org/10,3325/cmj.2019.60.405
  • Omaye, S.T., Turnbull, J.D., & Sauberlich, H.E. (1979). Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods in enzymology, 62, 3-11. Tietz, N.W. (1999). Text Book of Clinical Chemistry (3rd Ed.). Burtis C.A. and Ashwood.
  • Pinto, V. M., Poggi, M., Russo, R., Giusti, A., & Forni, G. L. (2019). Management of the aging beta-thalassemia transfusion-dependent population–The Italian experience. Blood reviews, 38, 100594.
  • Varley, H. (1967). Ramasays Dipyridyl method for total ironbinding capacity. In Practical Clinical Biochemistry, (pp. 475).
  • Varley, H., Gowenlock, A. & Bell, M., (1979). Practical clinical biochemistry: hormones, vitamins, drugs and poisons (5th ed.). Williams Heinemann Medical Books Ltd.
  • Al-Mosawy, W. F. (2017). The beta-thalassemia. Scientific Journal of Medical Research, 1(1),24-30.
  • Wierusz-Wysocka, B., Wysocki, H., Byks, H., Zozulińska, D., Wykrȩtowicz, A., & Kaźmierczak, M. (1995). Metabolic control quality and free radical activity in diabetic patients. Diabetes Research and Clinical Practice, 27(3), 193-197.
  • Chen, Y., Cai, N., Lai, Y., Xu, W., Li, J., Huang, L., ... & Chen, J. (2020). Thalidomide for the Treatment of Thrombocytopenia and Hypersplenism in Patients With Cirrhosis or Thalassemia. Frontiers in Pharmacology, 11, 1137. https://doi.org/10.3389/fphar.2020.01137

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Saba Z. AL-ABACHI This is me
University of Mosul
Palestine, State of


Sameer AL-GORANY This is me
Middle Technical University
Iraq


Abdulrazzaq ALTUWAIJARI This is me
Director of Diyala Haematology Center
Iraq


Jaafar GHAZY This is me
Philadelphia University
Jordan

Publication Date December 31, 2021
Published in Issue Year 2021, Volume 16, Issue

Cite

APA Z. Al-abachı, S. , Al-gorany, S. , Altuwaıjarı, A. & Ghazy, J. (2021). Measurements the Level of Lipid Peroxidation and Some Antioxidants in Blood Serum of Thalassemia’s Patients . The Eurasia Proceedings of Science Technology Engineering and Mathematics , 16 , 225-230 . DOI: 10.55549/epstem.1068616