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Investigation of the Role of Vitamin C In Enhancing the Activity of Antibacterial Agents and Biofilm Formation

Yıl 2021, Cilt: 16 , 195 - 203, 31.12.2021
https://doi.org/10.55549/epstem.1068610

Öz

Resistance to antibiotics is rapidly spreading across the globe, posing a new health-care challenge for all countries. Bacterial biofilms are three-dimensional formations made up of cells encased in a matrix of polymeric, making cells resistant to the drugs and the immune system. As a result, new tactics for inhibiting the production of the EPS matrix may lead to more efficient use of already available antibiotics. The mechanism of vitamins C effect on boosting the effectiveness of several anti-bacterial drugs was investigated in this study, the target isolates were obtained from University of Mosul/ Biology department/ bacterial culture collections and evaluated qualitatively and quantitatively. The isolates involved: Staphylococcus aureus, Escherichia coli ,Klebsiella sp., Serratia marcescens and Pseudomonas aeroginosa. Antibiotic sensitivity tests and biofilm producing assay results revealed that the majority of the isolates were resistant to a range of antibiotics and had a large capacity for biofilm formation when grown on a cover glass surface. Vitamin C is an antioxidant, a scavenger of active metabolites. The Minimum Inhibitory Concentration (MIC) of Vitamin C against selected isolates had been determined, and all further experiments used concentrations below the MIC. Our results showed that Vit.C pre-treatment enhance the bactericidal effect of antibiotic and increases bacterial susceptibility to antibiotics. Using light microscopy, experiments of sub inhibitory doses of Vitamin C revealed good suppression of selected isolates biofilm development on the cover glass surface. Vitamin C can be utilized as an antibiotic adjuvant in combination with antibiotic and has effective biofilm inhibition, caused by multidrug-resistant bacteria, according to evidence.

Kaynakça

  • Alves, M. J., Barreira, J. C., Carvalho, I., Trinta, L., Perreira, L., Ferreira, I. C., & Pintado, M. (2014). Propensity for biofilm formation by clinical isolates from urinary tract infections: developing a multifactorial predictive model to improve antibiotherapy. Journal of Medical Microbiology, 63(3), 471-477.
  • Bayer, A., Kirby, W., Sherris, J., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol, 45(4), 493-496.
  • Bjarnsholt, T., Tolker-Nielsen, T., Høiby, N., & Givskov, M. (2010). Interference of Pseudomonas aeruginosa signalling and biofilm formation for infection control. Expert Reviews in Molecular Medicine, 12, 1-11. doi:10.1017/S1462399410001420
  • Boissevain, C. H., & Spillane Jr, J. H. (1937). A note on the effect of synthetic ascorbic acid (vitamin C) on the growth of the tubercle bacillus. American Review of Tuberculosis, 35(5), 661-662.
  • Chen, X., Liu, R., Liu, X., Xu, C., & Wang, X. (2018). L-ascorbic Acid-2-Glucoside inhibits Helicobacter pylori-induced apoptosis through mitochondrial pathway in Gastric Epithelial cells. Biomedicine & Pharmacotherapy, 97, 75-81.
  • Cohen, M. L. (1992). Epidemiology of drug resistance: implications for a post-antimicrobial era. Science(Washington), 257(5073), 1050-1055.
  • Donlan, R. M., & Costerton, J. W. (2002). Biofilms: survival mechanisms of clinically relevant microorganisms. Clinical microbiology reviews, 15(2), 167-193.
  • Flores-Encarnación, M., Nava-Nolazco, R., Aguilar-Gutiérrez, G., Carreño-López, R., Carreno-L, & oopez, R. (2018). The effect of Thymus vulgaris on growth and biofilm formation of uropathogenic Escherichia coli. African Journal of Microbiology Research, 12(10), 237-242.
  • Foti, J. J., Devadoss, B., Winkler, J. A., Collins, J. J., & Walker, G. C. (2012). Oxidation of the guanine nucleotide pool underlies cell death by bactericidal antibiotics. Science, 336(6079), 315-319.
  • Gary, P. W. (2017). Koneman. Diagnostico Microbiologico/Microbiological diagnosis: Texto Y Atlas En Color/Text and Color Atlas.(Septima Ed.) Wolters Kluwer.
  • Gerceker, D., Karasartova, D., Elyürek, E., Barkar, S., KIyan, M., Özsan, T. M. & Sahin, F. (2009). A new, simple, rapid test for detection of DNase activity of microorganisms: DNase Tube test. The Journal of General and Applied Microbiology, 55(4), 291-294.
  • Guggenbichler, J. P., Assadian, O., Boeswald, M., & Kramer, A. (2011). Incidence and clinical implication of nosocomial infections associated with implantable biomaterials-catheters, ventilator-associated pneumonia, urinary tract infections. GMS Krankenhaushygiene interdisziplinär, 6(1), 1-19.
  • Hatano, T., Tsugawa, M., Kusuda, M., Taniguchi, S., Yoshida, T., Shiota, S., & Tsuchiya, T. (2008). Enhancement of antibacterial effects of epigallocatechin gallate, using ascorbic acid. Phytochemistry, 69(18), 3111-3116.
  • Helgadóttir, S., Pandit, S., Mokkapati, V. R., Westerlund, F., Apell, P., & Mijakovic, I. (2017). Vitamin C pretreatment enhances the antibacterial effect of cold atmospheric plasma. Frontiers in Cellular and Infection Microbiology, 7, 43.
  • Holloway, A. C., Gould, S. W., Fielder, M. D., Naughton, D. P., & Kelly, A. F. (2011). Enhancement of antimicrobial activities of whole and sub-fractionated white tea by addition of copper (II) sulphate and vitamin C against Staphylococcus aureus; a mechanistic approach. BMC Complementary and Alternative Medicine, 11(1), 1-9.
  • Jones, S. M., Dang, T. T., & Martinuzzi, R. (2009). Use of quorum sensing antagonists to deter the formation of crystalline Proteus mirabilis biofilms. International Journal of Antimicrobial Agents, 34(4), 360-364.
  • Khameneh, B., Diab, R., Ghazvini, K., & Bazzaz, B. S. F. (2016). Breakthroughs in bacterial resistance mechanisms and the potential ways to combat them. Microbial Pathogenesis, 95, 32-42.
  • Mai-Prochnow, A., Bradbury, M., Ostrikov, K., & Murphy, A. B. (2015). Pseudomonas aeruginosa biofilm response and resistance to cold atmospheric pressure plasma is linked to the redox-active molecule phenazine. PloS one, 10(6), e0130373.
  • Manjunath Mandhira Doss, M. R. (2013). Comparative evaluation of reversal of shear bond strength on bleached enamel using antioxidants–sodium ascorbate, salicylic acid & N-acetyl cysteine: An in vitro study (Doctoral dissertation, Tamilnadu Government Dental College and Hospital, Chennai).
  • Mansour, A., Manijeh, M., & Zohreh, P. (2009). Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics. Jundishapur Journal of Microbiology, 2(3), 118-123.
  • Martin-Cereceda, M., Alvarez, A. M., Serrano, S., & Guinea, A. (2001). Confocal and light microscope examination of protozoa and other microorganisms in the biofilms from a rotating biological contactor wastewater treatment plant. Acta Protozoologica, 40(4), 263-272.
  • McConkey, M., & Smith, D. T. (1933). The relation of vitamin C deficiency to intestinal tuberculosis in the guinea pig. The Journal of Experimental Medicine, 58(4), 503.
  • Mehmeti, I., Solheim, M., Nes, I. F., & Holo, H. (2013). Enterococcus faecalis grows on ascorbic acid. Applied and Environmental Microbiology, 79(15), 4756-4758.
  • Musthafa, K., Ravi, A., Annapoorani, A., Packiavathy, I. V., & Pandian, S. (2010). Evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy, 56(4), 333-339.
  • W. H. O. (2014). Global status report on noncommunicable diseases 2014: World Health Organization.
  • Pandit, S., Ravikumar, V., Abdel-Haleem, A. M., Derouiche, A., Mokkapati, V., Sihlbom, C., Westerlund, F. (2017). Low concentrations of vitamin C reduce the synthesis of extracellular polymers and destabilize bacterial biofilms. Frontiers in Microbiology, 8, 2599.
  • Puzari, M., Sharma, M., & Chetia, P. (2018). Emergence of antibiotic resistant Shigella species: A matter of concern. Journal of Infection and Public Health, 11(4), 451-454.
  • Roy, R., Tiwari, M., Donelli, G., & Tiwari, V. (2018). Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence, 9(1), 522-554.
  • Vilchèze, C., Hartman, T., Weinrick, B., & Jacobs, W. R. (2013). Mycobacterium tuberculosis is extraordinarily sensitive to killing by a vitamin C-induced Fenton reaction. Nature Communications, 4(1), 1-10.
  • Wei, J.-R., Horng, Y.-T., Lai, H.-C., Luh, K.-T., & Ho, S.-W. (2004). Effects of PNPG on cell growth cycle, motility machinery and quorum sensing in Serratia marcescens. Journal of microbiology, immunology, and infection= Wei mian yu gan ran za zhi, 37(1), 1-7.
Yıl 2021, Cilt: 16 , 195 - 203, 31.12.2021
https://doi.org/10.55549/epstem.1068610

Öz

Kaynakça

  • Alves, M. J., Barreira, J. C., Carvalho, I., Trinta, L., Perreira, L., Ferreira, I. C., & Pintado, M. (2014). Propensity for biofilm formation by clinical isolates from urinary tract infections: developing a multifactorial predictive model to improve antibiotherapy. Journal of Medical Microbiology, 63(3), 471-477.
  • Bayer, A., Kirby, W., Sherris, J., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol, 45(4), 493-496.
  • Bjarnsholt, T., Tolker-Nielsen, T., Høiby, N., & Givskov, M. (2010). Interference of Pseudomonas aeruginosa signalling and biofilm formation for infection control. Expert Reviews in Molecular Medicine, 12, 1-11. doi:10.1017/S1462399410001420
  • Boissevain, C. H., & Spillane Jr, J. H. (1937). A note on the effect of synthetic ascorbic acid (vitamin C) on the growth of the tubercle bacillus. American Review of Tuberculosis, 35(5), 661-662.
  • Chen, X., Liu, R., Liu, X., Xu, C., & Wang, X. (2018). L-ascorbic Acid-2-Glucoside inhibits Helicobacter pylori-induced apoptosis through mitochondrial pathway in Gastric Epithelial cells. Biomedicine & Pharmacotherapy, 97, 75-81.
  • Cohen, M. L. (1992). Epidemiology of drug resistance: implications for a post-antimicrobial era. Science(Washington), 257(5073), 1050-1055.
  • Donlan, R. M., & Costerton, J. W. (2002). Biofilms: survival mechanisms of clinically relevant microorganisms. Clinical microbiology reviews, 15(2), 167-193.
  • Flores-Encarnación, M., Nava-Nolazco, R., Aguilar-Gutiérrez, G., Carreño-López, R., Carreno-L, & oopez, R. (2018). The effect of Thymus vulgaris on growth and biofilm formation of uropathogenic Escherichia coli. African Journal of Microbiology Research, 12(10), 237-242.
  • Foti, J. J., Devadoss, B., Winkler, J. A., Collins, J. J., & Walker, G. C. (2012). Oxidation of the guanine nucleotide pool underlies cell death by bactericidal antibiotics. Science, 336(6079), 315-319.
  • Gary, P. W. (2017). Koneman. Diagnostico Microbiologico/Microbiological diagnosis: Texto Y Atlas En Color/Text and Color Atlas.(Septima Ed.) Wolters Kluwer.
  • Gerceker, D., Karasartova, D., Elyürek, E., Barkar, S., KIyan, M., Özsan, T. M. & Sahin, F. (2009). A new, simple, rapid test for detection of DNase activity of microorganisms: DNase Tube test. The Journal of General and Applied Microbiology, 55(4), 291-294.
  • Guggenbichler, J. P., Assadian, O., Boeswald, M., & Kramer, A. (2011). Incidence and clinical implication of nosocomial infections associated with implantable biomaterials-catheters, ventilator-associated pneumonia, urinary tract infections. GMS Krankenhaushygiene interdisziplinär, 6(1), 1-19.
  • Hatano, T., Tsugawa, M., Kusuda, M., Taniguchi, S., Yoshida, T., Shiota, S., & Tsuchiya, T. (2008). Enhancement of antibacterial effects of epigallocatechin gallate, using ascorbic acid. Phytochemistry, 69(18), 3111-3116.
  • Helgadóttir, S., Pandit, S., Mokkapati, V. R., Westerlund, F., Apell, P., & Mijakovic, I. (2017). Vitamin C pretreatment enhances the antibacterial effect of cold atmospheric plasma. Frontiers in Cellular and Infection Microbiology, 7, 43.
  • Holloway, A. C., Gould, S. W., Fielder, M. D., Naughton, D. P., & Kelly, A. F. (2011). Enhancement of antimicrobial activities of whole and sub-fractionated white tea by addition of copper (II) sulphate and vitamin C against Staphylococcus aureus; a mechanistic approach. BMC Complementary and Alternative Medicine, 11(1), 1-9.
  • Jones, S. M., Dang, T. T., & Martinuzzi, R. (2009). Use of quorum sensing antagonists to deter the formation of crystalline Proteus mirabilis biofilms. International Journal of Antimicrobial Agents, 34(4), 360-364.
  • Khameneh, B., Diab, R., Ghazvini, K., & Bazzaz, B. S. F. (2016). Breakthroughs in bacterial resistance mechanisms and the potential ways to combat them. Microbial Pathogenesis, 95, 32-42.
  • Mai-Prochnow, A., Bradbury, M., Ostrikov, K., & Murphy, A. B. (2015). Pseudomonas aeruginosa biofilm response and resistance to cold atmospheric pressure plasma is linked to the redox-active molecule phenazine. PloS one, 10(6), e0130373.
  • Manjunath Mandhira Doss, M. R. (2013). Comparative evaluation of reversal of shear bond strength on bleached enamel using antioxidants–sodium ascorbate, salicylic acid & N-acetyl cysteine: An in vitro study (Doctoral dissertation, Tamilnadu Government Dental College and Hospital, Chennai).
  • Mansour, A., Manijeh, M., & Zohreh, P. (2009). Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics. Jundishapur Journal of Microbiology, 2(3), 118-123.
  • Martin-Cereceda, M., Alvarez, A. M., Serrano, S., & Guinea, A. (2001). Confocal and light microscope examination of protozoa and other microorganisms in the biofilms from a rotating biological contactor wastewater treatment plant. Acta Protozoologica, 40(4), 263-272.
  • McConkey, M., & Smith, D. T. (1933). The relation of vitamin C deficiency to intestinal tuberculosis in the guinea pig. The Journal of Experimental Medicine, 58(4), 503.
  • Mehmeti, I., Solheim, M., Nes, I. F., & Holo, H. (2013). Enterococcus faecalis grows on ascorbic acid. Applied and Environmental Microbiology, 79(15), 4756-4758.
  • Musthafa, K., Ravi, A., Annapoorani, A., Packiavathy, I. V., & Pandian, S. (2010). Evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy, 56(4), 333-339.
  • W. H. O. (2014). Global status report on noncommunicable diseases 2014: World Health Organization.
  • Pandit, S., Ravikumar, V., Abdel-Haleem, A. M., Derouiche, A., Mokkapati, V., Sihlbom, C., Westerlund, F. (2017). Low concentrations of vitamin C reduce the synthesis of extracellular polymers and destabilize bacterial biofilms. Frontiers in Microbiology, 8, 2599.
  • Puzari, M., Sharma, M., & Chetia, P. (2018). Emergence of antibiotic resistant Shigella species: A matter of concern. Journal of Infection and Public Health, 11(4), 451-454.
  • Roy, R., Tiwari, M., Donelli, G., & Tiwari, V. (2018). Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence, 9(1), 522-554.
  • Vilchèze, C., Hartman, T., Weinrick, B., & Jacobs, W. R. (2013). Mycobacterium tuberculosis is extraordinarily sensitive to killing by a vitamin C-induced Fenton reaction. Nature Communications, 4(1), 1-10.
  • Wei, J.-R., Horng, Y.-T., Lai, H.-C., Luh, K.-T., & Ho, S.-W. (2004). Effects of PNPG on cell growth cycle, motility machinery and quorum sensing in Serratia marcescens. Journal of microbiology, immunology, and infection= Wei mian yu gan ran za zhi, 37(1), 1-7.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Khansa Mohammed Younıs

Ghaydaa M. A. Alı

Ashwaq Hazem Najem

Yayımlanma Tarihi 31 Aralık 2021
Yayımlandığı Sayı Yıl 2021Cilt: 16

Kaynak Göster

APA Mohammed Younıs, K., M. A. Alı, G., & Najem, A. H. (2021). Investigation of the Role of Vitamin C In Enhancing the Activity of Antibacterial Agents and Biofilm Formation. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 16, 195-203. https://doi.org/10.55549/epstem.1068610